Li Siyu, Duan Yuchen, Zhu Weigang, Cheng Shanshan, Hu Wenping
Key Laboratory of Organic Integrated Circuits, Ministry of Education, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China.
Adv Mater. 2024 Dec;36(49):e2412379. doi: 10.1002/adma.202412379. Epub 2024 Sep 10.
Organic thin film transistors (OTFTs) enable rapid and label-free high-sensitivity detection of target analytes due to their low cost, large-area processing, biocompatibility, and inherent signal amplification. At the same time, the freedom of synthesis, tailorability, and functionalization of organic semiconductor materials and their ability to be combined with flexible substrates make them one of the ideal platforms for biosensing. However, OTFTs-based biosensors still face significant challenges, such as unexpected surface adsorption, disordered conformation, inhomogeneous graft density, and flexibility of probe molecules that biological sensing probes would face during immobilization. In this review, efficient immobilization strategies based on OTFTs biological sensing probes developed in the last 5 years are highlighted. First, the biosensors are classified according to their sensing interface. Second, a comprehensive discussion of the types of biological sensing probes is presented. Third, three commonly used methods for immobilizing biological sensing probes and their challenges are briefly described. Finally, the applications of OTFTs-based biosensors for liquid phase detection are summarized. This review provides a comprehensive and timely review of optimization in sensing interface engineering so that efficient immobilization of biological sensing probes with sensing interfaces will contribute to the development of high-performance OTFTs-based biosensors.
有机薄膜晶体管（OTFT）因其低成本、大面积加工、生物相容性和固有的信号放大特性，能够实现对目标分析物的快速、无标记高灵敏度检测。同时，有机半导体材料合成的自由度、可定制性和功能化以及它们与柔性基板结合的能力，使其成为生物传感的理想平台之一。然而，基于OTFT的生物传感器仍面临重大挑战，例如生物传感探针在固定过程中会遇到的意外表面吸附、构象无序、接枝密度不均匀以及探针分子的灵活性等问题。在本综述中，重点介绍了过去5年中基于OTFT生物传感探针开发的高效固定策略。首先，根据传感界面将生物传感器进行分类。其次，对生物传感探针的类型进行了全面讨论。第三，简要描述了三种常用的固定生物传感探针的方法及其面临的挑战。最后，总结了基于OTFT的生物传感器在液相检测中的应用。本综述对传感界面工程的优化进行了全面且及时的回顾，以便通过传感界面高效固定生物传感探针将有助于高性能基于OTFT的生物传感器的发展。
