Li Min, Liu Rui, Chen Guopu, Wang Handong, Wang Jinglin, Kong Bin, Yu Chenjie
Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.
Department of Otolaryngology Head and Neck Surgery, Affiliated Nanjing Drum Tower Hospital Clinical College of Xuzhou Medical University, Nanjing 210008, China.
Research (Wash D C). 2024 Sep 9;7:0469. doi: 10.34133/research.0469. eCollection 2024.
Hydrogels have emerged as appealing prospects for wound healing due to their superior biocompatible qualities. However, the integration of antibacterial active substances into hydrogels for effective wound repair remains challenging. Here, we present a novel double-network hydrogel for nasal mucosal injury repair with antibacterial and self-healing capabilities. This hydrogel is the result of mixing aldehyde polyethylene glycol (PEG) and a carboxymethyl chitosan (CMCS)-based hydrogel with a photocured methylacrylate gelatin (GelMA) hydrogel to envelop mesenchymal stem cell exosomes (MSC-Exos). CMCS is rich in amino groups and facilitates antibacterial repair. Given the dynamically reversible Schiff base connections between the amino group of chitosan and the aldehyde group of modified PEG, the hydrogel can be easily injected into the lesion site because of its excellent injection and shear thinning properties. GelMA introduces an additional network layer for the hydrogel, which enhances its strength and extends the duration of stem cell exosomes on the wound surface. On the basis of these characteristics, we provide evidence that this compound hydrogel can substantially increase cell proliferation and regeneration, inhibit scar hyperplasia, and stimulate angiogenesis in rabbit nasal septum mucosa trauma models. These results suggest that MSC exosome-loaded hydrogels (ME-Gel) have substantial clinical potential for the repair and regeneration of nasal mucosa after surgery or trauma.
由于其优异的生物相容性，水凝胶已成为伤口愈合的有吸引力的前景。然而，将抗菌活性物质整合到水凝胶中以实现有效的伤口修复仍然具有挑战性。在此，我们提出了一种具有抗菌和自愈能力的新型双网络水凝胶用于鼻黏膜损伤修复。这种水凝胶是通过将醛基聚乙二醇（PEG）和基于羧甲基壳聚糖（CMCS）的水凝胶与光固化的甲基丙烯酸明胶（GelMA）水凝胶混合以包裹间充质干细胞外泌体（MSC-Exos）而制成的。CMCS富含氨基，有助于抗菌修复。鉴于壳聚糖的氨基与改性PEG的醛基之间存在动态可逆的席夫碱连接，该水凝胶因其出色的注射性和剪切变稀特性而可轻松注入病变部位。GelMA为水凝胶引入了额外的网络层，增强了其强度并延长了干细胞外泌体在伤口表面的停留时间。基于这些特性，我们提供证据表明这种复合水凝胶可以在兔鼻中隔黏膜创伤模型中显著增加细胞增殖和再生、抑制瘢痕增生并刺激血管生成。这些结果表明，负载MSC外泌体的水凝胶（ME-Gel）在手术或创伤后鼻黏膜的修复和再生方面具有巨大的临床潜力。
