Vorobyev Aleksey V, Khasin Yury I, Makarov Vasily V, Kirillov Sergey N, Verenchikov Anatoly N
Mass Spectrometry Consulting Ltd, MSC-CG, Bar, Montenegro.
Rapid Commun Mass Spectrom. 2024 Dec 15;38(23):e9909. doi: 10.1002/rcm.9909.
Gas chromatography-mass spectrometry (GC-MS) is a cornerstone technique for analyses of volatile and semivolatile compounds, constantly evolving to meet new challenges. The adoption of time-of-flight mass spectrometers (TOFMS) has enhanced GC-MS with faster spectral acquisition and broader mass coverage, enabling rapid GC separations. Multireflecting TOFMS (MRT) further improves resolution compared to traditional single-reflecting TOFMS, facilitating the detection of minor analytes within complex matrices. This paper presents a significant improvement in MRT sensitivity and dynamic range achieved through an ion beam compression within the ion guide and the use of a conventionally oriented orthogonal accelerator. Sensitivity is improved to 100 000 ion/pg at a resolution of R = 25,000, enabling the detection of low fg analyte quantities within 3 μg of matrix material into MS. The demonstrated linear dynamic range exceeding 6 orders of magnitude potentially enables even deeper exploration of complex mixtures with a bench top instrument.
气相色谱-质谱联用（GC-MS）是分析挥发性和半挥发性化合物的一项基础技术，它不断发展以应对新的挑战。飞行时间质谱仪（TOFMS）的采用提高了GC-MS的性能，实现了更快的光谱采集和更宽的质量覆盖范围，从而能够进行快速的GC分离。与传统的单反射TOFMS相比，多反射TOFMS（MRT）进一步提高了分辨率，有助于在复杂基质中检测痕量分析物。本文介绍了通过离子导向器内的离子束压缩和使用传统取向的正交加速器实现的MRT灵敏度和动态范围的显著提高。在分辨率R = 25,000时，灵敏度提高到100 000离子/皮克，能够在3微克基质材料中检测到低至飞克级的分析物进入质谱仪。所展示的超过6个数量级的线性动态范围，有可能使用台式仪器对复杂混合物进行更深入的探索。
