School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an 710061, China.
Food Chem. 2025 Jan 15;463(Pt 1):141153. doi: 10.1016/j.foodchem.2024.141153. Epub 2024 Sep 5.
Due to health hazards and co-contamination of mycotoxins, efficient separation and detection of multiple mycotoxins in food is highly desirable yet challenging. In this study, we prepared a novel mesoporous polypyrrole nanofiber mat (M-PPy NFM) for extracting multiple mycotoxins from food. The mesoporous effects and multifunctional PPy contribute to higher recovery and purification efficiency of M-PPy NFM for mycotoxins by facilitating hydrogen bonding and π-π interaction. Under optimized conditions, a simple, eco-friendly solid phase extraction (SPE) method coupled with high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) was developed for mycotoxin detection. This innovative method demonstrates good linearity (0.9991-0.9999), low detection limits (0.03-0.33 μg kg), satisfactory recoveries (92.0 %-108.0 %) and precision (0.3 %-11.7 %). Notably, it significantly reduces organic solvent consumption to 3.1 mL while minimizing adsorbent usage to 5.0 mg. Moreover, M-PPy NFM could be reused ten times. This study confirms the huge potential of M-PPy NFM for efficient applications in mycotoxin extraction and determination.
由于健康危害和真菌毒素的共同污染，高效分离和检测食品中的多种真菌毒素是非常理想的，但也是具有挑战性的。在本研究中，我们制备了一种新型介孔聚吡咯纳米纤维垫（M-PPy NFM），用于从食品中提取多种真菌毒素。介孔效应和多功能 PPy 通过促进氢键和π-π相互作用，有助于提高 M-PPy NFM 对真菌毒素的回收率和净化效率。在优化条件下，开发了一种简单、环保的固相萃取（SPE）方法，并结合高效液相色谱-质谱联用（HPLC-MS/MS）检测真菌毒素。这种创新方法表现出良好的线性（0.9991-0.9999）、低检测限（0.03-0.33 μg kg）、令人满意的回收率（92.0%-108.0%）和精密度（0.3%-11.7%）。值得注意的是，它将有机溶剂的消耗量显著减少到 3.1 mL，同时将吸附剂的用量减少到 5.0 mg。此外，M-PPy NFM 可以重复使用十次。本研究证实了 M-PPy NFM 在真菌毒素提取和测定中的高效应用具有巨大潜力。
