Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, School of Environmental Science and Engineering, Hainan University, Haikou, 570228, China.
State Key Laboratory of Marine Resources Utilization in the South China Sea, Hainan University, Haikou, 570228, China.
Nat Commun. 2024 Sep 10;15(1):7920. doi: 10.1038/s41467-024-52364-9.
Global urbanization requires more stable and sustainable wastewater treatment to reduce the burden on the water environment. To address the problem of substrate inhibition of microorganisms during wastewater treatment, which leads to unstable wastewater discharge, this study proposes an approach to enhance the tolerance of bacterial community by artificially setting up a non-lethal high substrate environment. And the feasibility of this approach was explored by taking the inhibition of anammox process by nitrite as an example. It was shown that the non-lethal high substrate environment could enhance the nitrite tolerance of anammox bacterial community, as the specific anammox activity increasing up to 24.71 times at high nitrite concentrations. Moreover, the system composed of anammox bacterial community with high nitrite tolerance also showed greater resistance (two-fold) in response to nitrite shock. The antifragility of the system was enhanced without affecting the operation of the main reactor, and the non-lethal high nitrite environment changed the dominant anammox genera to Candidatus Jettenia. This approach to enhance tolerance of bacterial community in a non-lethal high substrate environment not only allows the anammox system to operate stably, but also promises to be a potential strategy for achieving stable biological wastewater treatment processes to comply with standards.
全球城市化进程需要更加稳定和可持续的污水处理方式，以减轻对水环境的压力。为了解决污水处理过程中微生物基质抑制导致出水不稳定的问题，本研究提出了一种通过人为设置非致死性高基质环境来提高细菌群落耐受性的方法，并以亚硝酸盐抑制厌氧氨氧化过程为例，探讨了该方法的可行性。结果表明，非致死性高基质环境可以提高厌氧氨氧化菌群落对亚硝酸盐的耐受性，在高亚硝酸盐浓度下，特定厌氧氨氧化活性最高可提高 24.71 倍。此外，具有高亚硝酸盐耐受性的厌氧氨氧化菌群落组成的系统对亚硝酸盐冲击也表现出更大的抗性（两倍）。在不影响主反应器运行的情况下，提高了系统的反脆弱性，非致死性高亚硝酸盐环境改变了优势厌氧氨氧化菌属为杰滕斯氏菌属（Candidatus Jettenia）。该方法通过在非致死性高基质环境中提高细菌群落的耐受性，不仅可以使厌氧氨氧化系统稳定运行，而且有望成为实现稳定生物污水处理过程以符合标准的潜在策略。
