Dietzmann K, Kemnitz P, Schneidewind J
Acta Histochem. 1985;76(1):37-47.
Intralysosomal storage of polar lipids can be induced in rats by treatment with Chloroquine, which belongs to cationic amphiphilic compounds. The drug accumulates in lysosomes of cells of the peripheral nervous system and interferes with the catabolism of polar lipids. As a consequence the lysosomes are transformed into bodies which are characterized by an internal lamellated pattern. The lipids stored in the neuron cells are phospholipids. The purpose of the present study was to check up the chronological development of the structural and lipid histochemical alterations within neuron cells. A significant enlargement of perikarya was observed after treatment of 94 d. A moderate increase of lysosomes and phospholipids was noticed after 82 d. Some typical foam-cells were seen after a period of 250 d. Moreover, a special mitochondrial alteration was observed, which could express close correlations between phospholipid metabolism and damaged parts of mitochondria.
用属于阳离子两亲化合物的氯喹处理大鼠，可诱导极性脂质在溶酶体内蓄积。该药物积聚在周围神经系统细胞的溶酶体中，并干扰极性脂质的分解代谢。结果，溶酶体转化为具有内部层状结构特征的小体。储存在神经元细胞中的脂质是磷脂。本研究的目的是检查神经元细胞内结构和脂质组织化学改变的时间顺序发展。治疗94天后观察到核周体显著增大。82天后发现溶酶体和磷脂有中度增加。250天后可见一些典型的泡沫细胞。此外，还观察到一种特殊的线粒体改变，这可能表明磷脂代谢与线粒体受损部位之间存在密切关联。
