Kawabe Toshiaki, Kita Shota, Ohmura Isao, Michino Ryuji, Watanabe Hidenori, Sun Guanghao, Inoue Seiya
ARK Resource Co., Ltd, Kumamoto, Japan.
Kumamoto Industrial Research Institute, Kumamoto, Japan.
Lab Anim. 2024 Dec;58(6):591-601. doi: 10.1177/00236772241265541. Epub 2024 Sep 11.
The aim of this study was to verify the possibility of obtaining vital sign information using a laser and radar sensor in a manner that is non-invasive and painless for test animals. A dataset was obtained from respiratory movement of anaesthetized male F344 rats, signals of laser and radar sensors were recorded simultaneously with vital data acquired with an integrated multiple-channel intraoperative monitor. In addition, respiratory movements were also video recorded, and used as reference data of respiration rate (RR; ref-RR). Reference data for heart rate (HR; ref-HR) were obtained from the R wave of electrocardiogram data for each epoch. Signals recorded from the radar sensor (I- and Q-signals) were input to a computer, and HR (radar-HR) and RR (radar-RR) were estimated using the frequency analysis method. Among the six positions where respiratory movements were measured by the laser sensor, the number of peak counts matched the visual counts of respiratory movements in the video records. The respiratory movements were significantly the greatest over the most caudal rib in the dorsal ( < 0.001). The average radar-RR and ref-RR values showed correspondence (ref-RR, 69 ± 6.2 breaths/min; radar-RR, 68 ± 5.7 breaths/min ( = 0.04-1.00); equivalence ratio, 86%). The radar-HR data showed slight variability; however, there was 80% homology compared with the ref-HR values (ref-HR, 336 ± 19.6 beats/min; radar-HR, 348 ± 34.1 ( = 0.10-0.95)). Although comparison of the data under noradrenaline administration failed to track drug-induced changes in some cases, the HR and RR data of anesthetized rats measured from the radar sensor system showed comparable accuracy to other conventional methods.
本研究的目的是验证使用激光和雷达传感器以对实验动物无创且无痛的方式获取生命体征信息的可能性。从麻醉的雄性F344大鼠的呼吸运动中获取数据集，激光和雷达传感器的信号与通过集成多通道术中监测器获取的生命数据同时记录。此外，还对呼吸运动进行了视频记录，并用作呼吸频率（RR；参考RR）的参考数据。心率（HR；参考HR）的参考数据从每个时期的心电图数据的R波中获得。从雷达传感器记录的信号（I和Q信号）输入到计算机中，并使用频率分析方法估计HR（雷达-HR）和RR（雷达-RR）。在激光传感器测量呼吸运动的六个位置中，峰值计数的数量与视频记录中呼吸运动的视觉计数相匹配。呼吸运动在背部最尾侧肋骨处最为显著（<0.001）。雷达-RR和参考RR的平均值得出对应关系（参考RR，69±6.2次/分钟；雷达-RR，68±5.7次/分钟（=0.04-1.00）；等效比，86%）。雷达-HR数据显示出轻微的变异性；然而，与参考HR值相比具有80%的同源性（参考HR，336±19.6次/分钟；雷达-HR，348±34.1（=0.10-0.95））。尽管在去甲肾上腺素给药情况下的数据比较在某些情况下未能追踪药物引起的变化，但从雷达传感器系统测量的麻醉大鼠的HR和RR数据显示出与其他传统方法相当的准确性。
