Parvizi Alan Abolfazl, Ayen Esmail, Khaki Amir, Soleimanzadeh Ali
Department of Theriogenology and Poultry Disease, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Vet Res Forum. 2024;15(7):369-377. doi: 10.30466/vrf.2024.2000236.3852. Epub 2024 Jul 15.
During the freezing process of semen, due to the generating of significant amounts of free radicals, the quality of sperm changes. Epigallocatechin-3-gallate (EGCG) is a green tea catechin, which in this study was applied to investigate its effect on the quality of bulls' sperm. We collected semen samples with an artificial vagina from 12 Simmental bulls to evaluate the effect of EGCG (10.00 and 20.00 µmol) in two cryopreserving methods on the quality parameters of semen. We designed six groups including two control groups (method one and two) and four treatments (EGCG 10.00 µmol + method one; EGCG 20.00 µmol + method one; EGCG 10.00 µmol + method two; EGCG 20.00 µmol + method two). The 20.00 µmol EGCG and a method two significantly affected the amending oxidative conditions as well as an increase in total antioxidant capacity and a decrease in malondialdehyde. The effect of EGCG in both concentrations was more on method two. The desired impact on sperm motility, viability, inhibition of lipid peroxidation and sperm DNA damage was observed in EGCG groups compared to control groups. Among the two methods, the method two had fewer adverse effects on the plasma membrane, motility parameters, viability and DNA of sperm. The EGCG in the semen extender yielded a favorable impact on thawed sperm. This effect was prompted in combination with the method two.
在精液冷冻过程中，由于大量自由基的产生，精子质量会发生变化。表没食子儿茶素-3-没食子酸酯（EGCG）是一种绿茶儿茶素，在本研究中用于探究其对公牛精子质量的影响。我们用人工阴道从12头西门塔尔公牛采集精液样本，以评估两种冷冻保存方法中EGCG（10.00和20.00 μmol）对精液质量参数的影响。我们设计了六组，包括两个对照组（方法一和方法二）和四个处理组（EGCG 10.00 μmol + 方法一；EGCG 20.00 μmol + 方法一；EGCG 10.00 μmol + 方法二；EGCG 20.00 μmol + 方法二）。20.00 μmol的EGCG和方法二显著影响改善氧化条件以及提高总抗氧化能力和降低丙二醛水平。两种浓度的EGCG对方法二的影响更大。与对照组相比，EGCG组在精子活力、存活率、抑制脂质过氧化和精子DNA损伤方面有理想的效果。在两种方法中，方法二对精子质膜、活力参数、存活率和DNA的不良影响较少。精液稀释液中的EGCG对解冻后的精子产生了有利影响。这种效果在与方法二结合时更为明显。
