College of Life Science/Hebei Basic Science Center for Biotic Interactions, Institute of Life Science and Green Development, Hebei University, Baoding, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Microbiol Spectr. 2024 Oct 3;12(10):e0141224. doi: 10.1128/spectrum.01412-24. Epub 2024 Sep 11.
Microbiomes play crucial roles in insect adaptation, especially under stress such as pathogen invasion. Yet, how beneficial microbiomes assemble remains unclear. The wood-boring beetle , a major pest and vector of the pine wilt disease (PWD) nematode, offers a unique model. We conducted controlled experiments using amplicon sequencing (16S rRNA and ITS) within galleries where beetles and microbes interact. PWD significantly altered bacterial and fungal communities, suggesting distinct assembly processes. Deterministic factors like priority effects, host selection, and microbial interactions shaped microbiome composition, distinguishing healthy from PWN-infected galleries. Actinobacteria, Firmicutes, and Ophiostomataceae emerged as potentially beneficial, aiding beetle's development and pathogen resistance. This study unveils how nematode-induced changes in gallery microbiomes influence beetle's development, shedding light on microbiome assembly amid insect-pathogen interactions. Insights gleaned enhance understanding of PWD spread and suggest novel management strategies via microbiome manipulation.IMPORTANCEThis study explores the assembly process of gallery microbiomes associated with a wood-boring beetles, , a vector of the pine wilt disease (PWD). By conducting controlled comparison experiments and employing amplicon approaches, the study reveals significant changes in taxonomic composition and functional adaptation of bacterial and fungal communities induced by PWD. It identifies deterministic processes, including priority effects, host selection, and microbial interactions, as major drivers in microbiome assembly. Additionally, the study highlights the presence of potentially beneficial microbes such as Actinobacteria, Firmicutes, and Ophiostomataceae, which could enhance beetle development and resistance to pathogens. These findings shed light on the intricate interplay among insects, microbiomes, and pathogens, contributing to a deeper understanding of PWD prevalence and suggesting innovative management strategies through microbiome manipulation.
微生物组在昆虫适应中起着至关重要的作用，尤其是在受到病原体入侵等压力的情况下。然而，有益微生物组的组装方式仍不清楚。钻蛀性甲虫是松材线虫病（PWD）的主要害虫和媒介，为我们提供了一个独特的模型。我们在甲虫和微生物相互作用的坑道内进行了受控实验，采用扩增子测序（16S rRNA 和 ITS）。PWD 显著改变了细菌和真菌群落，表明存在不同的组装过程。优先效应、宿主选择和微生物相互作用等确定性因素塑造了微生物组的组成，将健康坑道和感染 PWN 的坑道区分开来。放线菌、厚壁菌门和奥霉囊菌科的出现可能具有有益作用，有助于甲虫的发育和对病原体的抗性。这项研究揭示了线虫诱导的坑道微生物组变化如何影响甲虫的发育，为昆虫-病原体相互作用中的微生物组组装提供了新的认识。研究结果增强了对 PWD 传播的理解，并通过微生物组操纵提出了新的管理策略。
本研究探讨了与钻蛀性甲虫相关的坑道微生物组的组装过程，该甲虫是松材线虫病（PWD）的媒介。通过进行对照比较实验和使用扩增子方法，研究揭示了 PWD 诱导的细菌和真菌群落的分类组成和功能适应性的显著变化。确定了优先效应、宿主选择和微生物相互作用等确定性过程是微生物组组装的主要驱动因素。此外，研究还强调了一些潜在有益微生物的存在，如放线菌、厚壁菌门和奥霉囊菌科，它们可以增强甲虫的发育和对病原体的抗性。这些发现揭示了昆虫、微生物组和病原体之间复杂的相互作用，有助于更深入地了解 PWD 的流行情况，并通过微生物组操纵提出了创新的管理策略。
