Wu Xue-Qin, Chen Xiu-Mei, Pan Yi-Yu, Sun Chuang, Tian Jia-Xin, Qian Ai-Dong, Niu Xiao-Tian, Kong Yi-di, Li Min, Wang Gui-Qin
College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Key Laboratory for Animal Production, Product Quality and Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Key Laboratory for Animal Production, Product Quality and Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Fish Shellfish Immunol. 2024 Sep;152:109775. doi: 10.1016/j.fsi.2024.109775. Epub 2024 Jul 16.
Bacterial intestinal inflammation frequently occurs in cultured fish. Nevertheless, research on intestinal barrier dysfunction in the process of intestinal inflammation is deficient. In this study, we explored the changes of intestinal inflammation induced by Aeromonas hydrophila (A. hydrophila) in snakehead and the relationship between intestinal barrier and inflammation. Snakehead [(13.05 ± 2.39) g] were infected via anus with A. hydrophila. Specimens were collected for analysis at 0, 1, 3, 7 and 21 d post-injection. The results showed that with the increase of exposure time, the hindgut underwent stages of normal function, damage, damage deterioration, repair and recovery. Relative to 0 d, the levels of IL-1β and TNF-α in serum, and the expression of nod1, tlr1, tlr5, nf-κb, tnf-α and il-1β in intestine were significantly increased, and showed an upward then downward pattern over time. However, the expression of tlr2 and il-10 were markedly decreased, and showed the opposite trend. In addition, with the development of intestinal inflammation, the diversity and richness of species, and the levels of phylum and genus in intestine were obviously altered. The levels of trypsin, LPS, AMS, T-SOD, CAT, GPx, AKP, LZM and C3 in intestine were markedly reduced, and displayed a trend of first decreasing and then rebounding. The ultrastructure observation showed that the microvilli and tight junction structure of intestinal epithelial cells experienced normal function initially, then damage, and finally recovery over time. The expression of claudin-3 and zo-1 in intestine were significantly decreased, and showed a trend of first decreasing and then rebounding. Conversely, the expression of mhc-i, igm, igt and pigr in intestine were markedly increased, and displayed a trend of increasing first and then decreasing. The above results revealed the changes in intestinal barrier during the occurrence and development of intestinal inflammation, which provided a theoretical basis for explaining the relationship between the two.
细菌性肠道炎症在养殖鱼类中频繁发生。然而，关于肠道炎症过程中肠道屏障功能障碍的研究尚显不足。在本研究中，我们探究了嗜水气单胞菌（A. hydrophila）诱导乌鳢肠道炎症的变化以及肠道屏障与炎症之间的关系。将体重为[(13.05 ± 2.39) g]的乌鳢经肛门感染嗜水气单胞菌。在注射后0、1、3、7和21天采集样本进行分析。结果表明，随着暴露时间的增加，后肠经历了正常功能、损伤、损伤恶化、修复和恢复阶段。相对于0天，血清中IL-1β和TNF-α水平以及肠道中nod1、tlr1、tlr5、nf-κb、tnf-α和il-1β的表达显著增加，并随时间呈先上升后下降的模式。然而，tlr2和il-10的表达明显降低，呈现相反趋势。此外，随着肠道炎症的发展，肠道中物种的多样性和丰富度以及门和属的水平明显改变。肠道中胰蛋白酶、LPS、AMS、T-SOD、CAT、GPx、AKP、LZM和C3的水平显著降低，并呈现先下降后反弹的趋势。超微结构观察表明，肠道上皮细胞的微绒毛和紧密连接结构最初功能正常，随后受损，最终随时间恢复。肠道中claudin-3和zo-1的表达显著降低，并呈现先下降后反弹的趋势。相反，肠道中mhc-i、igm、igt和pigr的表达明显增加，并呈现先增加后降低的趋势。上述结果揭示了肠道炎症发生和发展过程中肠道屏障的变化，为解释两者之间的关系提供了理论依据。
