State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Ecotoxicol Environ Saf. 2024 Oct 1;284:117007. doi: 10.1016/j.ecoenv.2024.117007. Epub 2024 Sep 10.
Aedes albopictus, a common mosquito in Zhejiang Province, is a carrier of more than twenty arboviruses. There are dozens or even hundreds of imported cases of dengue fever every year in Zhejiang Province, and there have also been many local outbreaks caused by imported cases of dengue fever. The objectives were to assess the resistance of larvae and adults of several Ae. albopictus strains in Zhejiang Province to commonly used pyrethroid insecticides (beta-cypermethrin, deltamethrin and permethrin), and detect mutations in the sodium channel gene, to further analyse the relationship between phenotypic resistance and the frequency of mutations. The resistance of eight field strains of Ae. albopictus larvae to beta-cypermethrin, deltamethrin and permethrin ranged from 8.17 to 36.06, 12.12-107.3 and 1.55-81.9, respectively, and there was a significant positive correlation of interaction resistance among the three insecticides. The mutation frequencies of I1532T and F1534S in the larvae of Ae. albopictus were 0-6.25 % and 42.19-100.00 %. Moreover, the diagnostic doses of the three pyrethroids for adult Ae. albopictus mosquitoes were 0.2510 g/L, 0.1562 g/L, and 0.9072 g/L. Except for the Zhoushan strain, which was suspected to be resistant to beta-cypermethrin, the other field strains were resistant to the three pyrethroids, and there was a significant positive correlation of cross-resistance among the three insecticides. The mutation frequencies of I1532T and F1534S of adult Ae. albopictus were 0-1.56 % and 62.50-100.00 %. In addition, the LC of the larvae and the mortality rate of adult Ae. albopictus after treatment with the three pyrethroids were significantly and positively correlated with the frequency of the F1534S mutation. F1534S mutation occurred earlier than I1532T mutation in both larvae and adult Ae. albopictus. F1534S mutation in the sodium channel gene may be a particular biomolecular detection marker for resistance to pyrethroid insecticides in Ae. albopictus in Zhejiang Province.
浙江省常见的白纹伊蚊是二十多种虫媒病毒的载体。浙江省每年有数十甚至数百例输入性登革热病例，也有许多由输入性登革热引起的本地暴发。本研究旨在评估浙江省几种白纹伊蚊幼虫和成虫对常用拟除虫菊酯类杀虫剂（β-氯氰菊酯、溴氰菊酯和氯菊酯）的抗性，并检测钠离子通道基因突变，进一步分析表型抗性与突变频率之间的关系。8 株白纹伊蚊幼虫对β-氯氰菊酯、溴氰菊酯和氯菊酯的抗性分别为 8.17-36.06、12.12-107.3 和 1.55-81.9，三种杀虫剂之间存在显著的交互抗性正相关。白纹伊蚊幼虫 I1532T 和 F1534S 的突变频率为 0-6.25%和 42.19-100.00%。此外，三种拟除虫菊酯对成蚊的诊断剂量分别为 0.2510 g/L、0.1562 g/L 和 0.9072 g/L。除舟山株疑似对β-氯氰菊酯产生抗药性外，其他田间分离株对三种拟除虫菊酯均有抗药性，三种杀虫剂之间存在显著的正交互抗性。白纹伊蚊成虫 I1532T 和 F1534S 的突变频率为 0-1.56%和 62.50-100.00%。此外，幼虫的 LC 和三种拟除虫菊酯处理后成蚊的死亡率与 F1534S 突变频率呈显著正相关。在白纹伊蚊幼虫和成虫中，F1534S 突变先于 I1532T 突变发生。钠离子通道基因 F1534S 突变可能是浙江省白纹伊蚊对拟除虫菊酯类杀虫剂抗性的特定生物分子检测标志物。
