中国白纹伊蚊（双翅目：蚊科）野外种群成蚊对拟除虫菊酯的抗性与击倒抗性（kdr）突变的相关性。
Correlation between adult pyrethroid resistance and knockdown resistance (kdr) mutations in Aedes albopictus (Diptera: Culicidae) field populations in China.
机构信息
Department of Tropical Infectious Diseases, Second Military Medical University, Shanghai, 200433, China.
Institute of Disease Control and Prevention, People's Liberation Army of China, Beijing, 100071, China.
出版信息
Infect Dis Poverty. 2018 Sep 4;7(1):86. doi: 10.1186/s40249-018-0471-y.
BACKGROUND
Arboviral disease transmitted by Aedes albopictus such as dengue fever is an important threat to human health. Pyrethroid resistance raises a great challenge for mosquito control. A systematic assessment of Ae. albopictus resistance status in China is urgently needed, and the study of correlation between pyrethroid resistance and knockdown resistance (kdr) mutations would provide information to guide the control of the Ae. albopictus vector.
METHODS
Five field populations of Ae. albopictus were collected from Jinan (JN), Hangzhou (HZ), Baoshan (BS), Yangpu (YP) and Haikou (HK), China in 2017. Insecticide-impregnated papers were prepared with four pyrethroid chemicals, deltamethrin, permethrin, beta-cypermethrin and lambda-cyhalothrin. The susceptibility of Ae. albopictus to pyrethroids was tested by the WHO tube assay. Kdr mutations were identified by PCR and sequencing. Moreover, the correlation analysis between kdr alleles and pyrethroid resistance was performed.
RESULTS
All five populations of Ae. albopictus showed resistance to four pyrethroid insecticides. One kdr mutant allele at codon 1532 and three at 1534 were detected with frequency of 5.33% (I1532T), 44.20% (F1534S), 1.83% (F1534 L) and 0.87% (F1534C), respectively. Both 1532 and 1534 mutation mosquitoes were found in the BS and YP populations. Allele I1532T was negatively correlated with deltamethrin resistance phenotype (OR < 1), while F1534S mutation was positively correlated with deltamethrin and permethrin resistance (OR > 1).
CONCLUSIONS
The five field populations of Ae. albopictus adults were all resistant to deltamethrin, permethrin, beta-cypermethrin and lambda-cyhalothrin. Mutant F1534S was clearly associated with pyrethroid resistance phenotype in Ae. albopictus and this could be developed as a molecular marker to monitor the pyrethroid resistance problem in China.
背景
白纹伊蚊传播的虫媒病毒病，如登革热，对人类健康构成了重大威胁。拟除虫菊酯类杀虫剂的抗性极大地增加了蚊虫控制的难度。因此，迫切需要对中国白纹伊蚊的抗性状况进行系统评估，同时，对其与击倒抗性（kdr）突变相关的研究将为指导白纹伊蚊媒介的控制提供信息。
方法
2017 年，从中国济南（JN）、杭州（HZ）、保山（BS）、杨浦（YP）和海口（HK）采集了五个白纹伊蚊野外种群。使用四种拟除虫菊酯类杀虫剂（溴氰菊酯、氯菊酯、高效氯氰菊酯和高效氯氟氰菊酯）制备了浸渍纸片。采用 WHO 管法测试白纹伊蚊对拟除虫菊酯类杀虫剂的敏感性。通过 PCR 和测序鉴定 kdr 突变。此外，还进行了 kdr 等位基因与拟除虫菊酯类杀虫剂抗性的相关性分析。
结果
五个白纹伊蚊种群均对四种拟除虫菊酯类杀虫剂表现出抗性。在 1532 位密码子检测到一个 kdr 突变等位基因（I1532T），在 1534 位密码子检测到三个突变等位基因（F1534S、F1534L 和 F1534C），频率分别为 5.33%、44.20%、1.83%和 0.87%。BS 和 YP 种群中同时存在 1532 和 1534 突变蚊。1532 位突变的等位基因 I1532T 与溴氰菊酯的抗性表型呈负相关（OR<1），而 1534 位突变的等位基因 F1534S 与溴氰菊酯和氯菊酯的抗性表型呈正相关（OR>1）。
结论
五个白纹伊蚊野外种群对溴氰菊酯、氯菊酯、高效氯氰菊酯和高效氯氟氰菊酯均表现出抗性。突变体 F1534S 与白纹伊蚊对拟除虫菊酯类杀虫剂的抗性表型明显相关，可作为监测中国拟除虫菊酯类杀虫剂抗性问题的分子标记。
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