School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, PR China.
Department of Printing and Packing Engineering, Shanghai Publishing and Printing College, Shanghai, 200093, PR China.
Talanta. 2025 Jan 1;281:126866. doi: 10.1016/j.talanta.2024.126866. Epub 2024 Sep 10.
A highly efficient electrochemical aptamer sensor for the detection of tetracycline (TC) was prepared by using SnC@Au@Apta. Metal tin has good electrochemical activity and high conductivity. It is often used as an electrochemical sensing material. The nanofibers prepared by electrospinning machine make the metal distribution more uniform, not easy to agglomerate, and have a certain porosity, which can improve the sensitivity of sensor detection. Carbonization further enhances conductivity. The gold nanoparticles (AuNPs) on the surface of SnC nanomaterials improve the electrochemical detection performance, and also act as the binding site of the TC aptamer, which is stably combined with the thiol group at the end of the TC aptamer. The TC aptamer specifically binds to TC to detect TC in the sample. The electrochemical performance of SnC@Au@Apta was evaluated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, the detection range of SnC@Au@Apta is wide (0.001-100 μM), the detection limit is low (0.83 nM), and it has excellent selectivity, stability and reproducibility. In addition, SnC@Au@Apta can be used to detect TC in milk samples.
一种用于检测四环素（TC）的高效电化学适体传感器是通过使用 SnC@Au@Apta 制备的。金属锡具有良好的电化学活性和高导电性。它通常被用作电化学传感材料。静电纺丝机制备的纳米纤维使金属分布更加均匀，不易团聚，并且具有一定的孔隙率，这可以提高传感器检测的灵敏度。碳化进一步增强了导电性。SnC 纳米材料表面的金纳米粒子（AuNPs）提高了电化学检测性能，并且还充当 TC 适体的结合位点，与 TC 适体末端的硫醇基团稳定结合。TC 适体特异性结合 TC 以检测样品中的 TC。通过循环伏安法（CV）和差分脉冲伏安法（DPV）评估了 SnC@Au@Apta 的电化学性能。在最佳条件下，SnC@Au@Apta 的检测范围很宽（0.001-100 μM），检测限很低（0.83 nM），并且具有出色的选择性、稳定性和重现性。此外，SnC@Au@Apta 可用于检测牛奶样品中的 TC。
