State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Puyang Academy of Agriculture and Forestry Sciences, China.
Int J Biol Macromol. 2024 Nov;279(Pt 4):135518. doi: 10.1016/j.ijbiomac.2024.135518. Epub 2024 Sep 10.
Trehalose-6-phosphate synthase (TPS) genes play an active role in the trehalose metabolism pathway that regulates the responses of plants to diverse stresses. However, the functional identification, comparison, and conservatism of TPS genes in the responses of woody plants, especially poplars, to drought stress remain unclear. Here, the trehalose content of 84K (Populus alba × P. glandulosa) poplars was down-regulated and PagTPS and PagTPP genes had diverse response patterns under drought stress. Physicochemical properties, expression patterns, and functions of PagTPS1 and PagTPS10, two class I members of TPS gene family, were identified and compared. Transgenic 84K poplars overexpressing PagTPS1 and PagTPS10 had significantly higher trehalose content with approximately 138% and 123%, respectively, and stronger drought tolerance compared to WT. PagTPS1 and PagTPS10 promoted the expression of TPPA genes and drought-responsive genes. Accordingly, poplars inhibiting PagTPS1 and PagTPS10 expression via RNA interference had lower trehalose content and drought tolerance. Simultaneously, overexpressing PagTPS1 and PagTPS10 improved the trehalose content and drought tolerance of Arabidopsis. Overall, we proposed a model of the effects of PagTPS1 and PagTPS10 as conservative regulators on the responses of plants to drought, which would provide new insights into the functional explorations of TPS genes in plants.
海藻糖-6-磷酸合酶（TPS）基因在调节植物对各种胁迫响应的海藻糖代谢途径中发挥着积极作用。然而，木本植物，特别是杨树，对干旱胁迫响应中 TPS 基因的功能鉴定、比较和保守性仍不清楚。在这里，84K（白杨×白杨）杨树的海藻糖含量下调，PagTPS 和 PagTPP 基因在干旱胁迫下表现出不同的响应模式。鉴定和比较了 TPS 基因家族的两个 I 类成员 PagTPS1 和 PagTPS10 的理化性质、表达模式和功能。过表达 PagTPS1 和 PagTPS10 的转基因 84K 杨树的海藻糖含量分别显著提高了约 138%和 123%，与 WT 相比具有更强的耐旱性。PagTPS1 和 PagTPS10 促进了 TPPA 基因和干旱响应基因的表达。因此，通过 RNA 干扰抑制 PagTPS1 和 PagTPS10 表达的杨树的海藻糖含量和耐旱性较低。同时，过表达 PagTPS1 和 PagTPS10 提高了拟南芥的海藻糖含量和耐旱性。总体而言，我们提出了 PagTPS1 和 PagTPS10 作为保守调节剂对植物响应干旱胁迫的影响模型，为植物 TPS 基因的功能探索提供了新的见解。
