State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, College of Chemistry and Chemical Engineering, Ningxia University, Yinchuan, China.
Luminescence. 2024 Sep;39(9):e4900. doi: 10.1002/bio.4900.
Glucose level is an important indicator of diabetes, and maintaining an appropriate physiological concentration of glucose is important for human health. However, traditional optical sensors are interfered by the interference of strong background autofluorescence and natural responsive luminescence, which severely limits their application in complex biological samples. Herein, as a novel glucose biosensing probe, green-emitting ZnGeO:Mn, Eu (ZGME) persistent luminescence nanoparticles (PLNPs) with pH stimulus-responsive was prepared by a facile one-pot hydrothermal method. We also investigated the pH stimulus-responsive luminescence behaviour of ZGME over a range of pH values from 2.8 to 8.0. Taking advantage of the interesting property that ZGME photoluminescence intensity has a pH response, within an extraordinarily narrow pH range of 5.0-6.5 for highly selectivity and sensitive determination of glucose level in human samples by acid-responsive quenching and persistent luminescent performance. The detection results show high accuracy of the measured values of glucose in serum with a wide detection range (2.5 μg L-10 mg L) and low detection limit (0.5 μg L). Finally, the pH-responsive persistent luminescence also makes ZGME promising for high-level fingerprint information encryption. Hence, the established pH stimulation-responsive PLNPs-based biosensing probe offers excellent performance with high selective, accuracy and signal-to-noise ratio for detection of glucose level in human samples.
血糖水平是糖尿病的一个重要指标，维持适当的生理葡萄糖浓度对人体健康很重要。然而，传统的光学传感器受到强背景自发荧光和自然响应发光的干扰，严重限制了它们在复杂生物样本中的应用。在此，我们通过一种简便的一步水热法制备了具有 pH 刺激响应的绿色发射 ZnGeO:Mn，Eu（ZGME）持续发光纳米粒子（PLNPs）作为新型葡萄糖生物传感探针。我们还研究了 ZGME 在 pH 值为 2.8 到 8.0 的范围内的 pH 刺激响应发光行为。利用 ZGME 光致发光强度具有 pH 响应的有趣特性，在极其狭窄的 pH 范围内（5.0-6.5），通过酸响应猝灭和持续发光性能对人样中葡萄糖水平进行高度选择性和灵敏的测定。检测结果表明，该传感器在血清中葡萄糖的测量值具有很高的准确性，检测范围宽（2.5μg/L-10mg/L），检测限低（0.5μg/L）。最后，pH 响应的持续发光也使得 ZGME 有望用于高等级指纹信息加密。因此，所建立的基于 pH 刺激响应的 PLNPs 生物传感探针在检测人样中的葡萄糖水平方面具有出色的性能，具有高选择性、准确性和信噪比。
