CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences , Fuzhou, Fujian 350002, China.
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
ACS Nano. 2017 Aug 22;11(8):8185-8191. doi: 10.1021/acsnano.7b03128. Epub 2017 Jul 5.
Persistent luminescence nanoparticles (PLNPs), which can remain luminescent after cessation of excitation, have emerged as important materials in biomedicine due to their special ability to eliminate tissue autofluorescence. Even though significant advances have been made in bioimaging, studies on controlled synthesis of PLNPs with tunable properties are lacking. Until now, only a few studies have reported the synthesis of quasi-spherical ZnGaO:Cr PLNPs, and direct synthesis of PLNPs with other shapes and chemical compositions has not been reported. Herein, we report the direct synthesis of ZnGeO:Mn (ZGO:Mn) persistent luminescence nanorods (NRs). The length and persistent luminescence of ZGO:Mn NRs can be fine-tuned by simply changing the pH of the hydrothermal reaction system. Moreover, ZGO:Mn NRs exhibit rapid growth rate, and NRs with strong persistent luminescence can be obtained within 30 min of hydrothermal treatment. Aptamer-guided ZGO:Mn bioprobes were further constructed and applied to serum lysozyme analysis. Serum samples from patients with lung cancer, gastric cancer, and colorectal cancer were collected, and the concentrations of lysozyme in these samples were determined. Since the bioprobes displayed long persistent luminescence, serum autofluorescence interference was completely eliminated. The lysozyme quantification results were in good agreement with those obtained using a clinical method, suggesting the good potential of the bioprobes in the analysis of clinical samples. The developed ZGO:Mn NRs possess tunable length and persistent luminescence, and they are ideal for eliminating autofluorescence interference in biosensing, making them valuable in research areas such as studying the functions of biomolecules and monitoring of molecular/cellular networks in their native contexts.
持续发光纳米粒子（PLNPs）在停止激发后仍能发光，由于其消除组织自发荧光的特殊能力，已成为生物医学中的重要材料。尽管在生物成像方面取得了重大进展，但对于具有可调特性的 PLNPs 的可控合成研究仍缺乏。到目前为止，只有少数研究报道了准球形 ZnGaO:Cr PLNPs 的合成，而具有其他形状和化学成分的 PLNPs 的直接合成尚未报道。在此，我们报告了 ZnGeO:Mn（ZGO:Mn）持续发光纳米棒（NRs）的直接合成。通过简单改变水热反应体系的 pH 值，可以精细调节 ZGO:Mn NRs 的长度和持续发光。此外，ZGO:Mn NRs 表现出快速的生长速率，在水热处理 30 分钟内即可获得具有强持续发光的 NRs。进一步构建了适体引导的 ZGO:Mn 生物探针，并将其应用于血清溶菌酶分析。收集了来自肺癌、胃癌和结直肠癌患者的血清样本，并测定了这些样本中溶菌酶的浓度。由于生物探针显示出长持续发光，因此完全消除了血清自发荧光的干扰。溶菌酶定量结果与临床方法获得的结果吻合良好，表明生物探针在临床样本分析中具有良好的应用潜力。开发的 ZGO:Mn NRs 具有可调的长度和持续发光，非常适合消除生物传感中的自发荧光干扰，使其在研究生物分子功能和监测分子/细胞网络在其天然环境中的作用等领域具有重要价值。
