Jiangxi Province Key Laboratory of Organic Functional Molecules, Institute of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.
YuZhang Normal University, Nanchang, 330013, PR China.
Anal Bioanal Chem. 2024 Nov;416(26):5753-5762. doi: 10.1007/s00216-024-05494-4. Epub 2024 Sep 11.
As a kind of antibiotic, tetracycline (TC) might remain in animal blood and milk products during use, which poses a risk to humans after consumption. Therefore, a ratiometric fluorescence probe was proposed for the detection of TC, which was based on an Eu functionalized hydrogen-bonded organic framework (HOF). Since there are a large number of N and O atoms in the skeleton of HOF, more Eu could be loaded onto HOF by forming coordinate bonds, while preserving the fluorescence of luminol monomer in HOF. In the presence of TC, the fluorescence of luminol monomer was attenuated at 425 nm due to inner filter effect (IFE), while TC selectively enhanced the fluorescence peak at 617 nm of Eu under the influence of antenna effect (AE). This highly sensitive probe could detect TC in the range of 0.1-60 μM and had a low limit of detection of 8.51 nM. Besides, the HOF@Eu probe was able to detect TC in actual samples (milk and tap water) with good recoveries (95.09%-111.51%) and precision (R < 4.78%), indicating this probe has great application potential for the detection of TC in food.
作为一种抗生素，四环素（TC）在使用过程中可能会残留在动物血液和奶制品中，人类食用后会对其造成风险。因此，我们提出了一种基于铕功能化氢键有机框架（HOF）的比率荧光探针来检测 TC。由于 HOF 的骨架中含有大量的 N 和 O 原子，因此可以通过形成配位键将更多的 Eu 负载到 HOF 上，同时保持 HOF 中鲁米诺单体的荧光。在 TC 的存在下，由于内滤效应（IFE），鲁米诺单体的荧光在 425nm 处被减弱，而在天线效应（AE）的影响下，TC 选择性地增强了 Eu 的荧光峰在 617nm 处。这个高灵敏度的探针可以在 0.1-60μM 的范围内检测 TC，检测限低至 8.51nM。此外，HOF@Eu 探针能够在实际样品（牛奶和自来水）中检测 TC，回收率良好（95.09%-111.51%），精密度（R<4.78%），表明该探针在食品中检测 TC 具有很大的应用潜力。
