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异养硝化-好氧反硝化菌在膜生物反应器(MBR)中的应用研究。

Research on the application of heterotrophic nitrification-aerobic denitrification bacteria in membrane bioreactor (MBR).

机构信息

School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China.

School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.

出版信息

Biotechnol Lett. 2024 Dec;46(6):1013-1025. doi: 10.1007/s10529-024-03529-5. Epub 2024 Sep 11.

DOI:10.1007/s10529-024-03529-5
PMID:39261356
Abstract

Inoculating heterotrophic nitrification-aerobic denitrification bacteria (HN-AD) to enhance membrane bioreactor (MBR) efficiency may result in the loss of functional bacteria. Therefore, this study compares the application results of enhancing MBR with a self-designed biological amplifier coupled with HN-AD against the performance of conventional MBR. After enhancement, the MBR achieved a removal efficiency of 96.7% for NH-N (100 mg/L) and 96.4% for COD (400 mg/L) in synthetic wastewater. There was a 33% increase in TN (100 mg/L) removal efficiency. The dominant bacteria in the MBR were Alcaligenes (48.4%) and Thauera (15.2%). Additionally, the abundance of denitrification genes (nirK, norB, nosZ) increased in the enhanced MBR, contributing to improved TN removal efficiency. The use of a biological amplifier effectively solved the problem of HN-AD loss in sewage treatment.

摘要

接种异养硝化-好氧反硝化菌(HN-AD)以提高膜生物反应器(MBR)效率可能导致功能菌的损失。因此,本研究比较了通过自行设计的生物增效器与 HN-AD 增强 MBR 的应用效果与传统 MBR 的性能。增强后,MBR 对合成废水中的 NH-N(100mg/L)和 COD(400mg/L)的去除效率达到了 96.7%和 96.4%,TN(100mg/L)的去除效率提高了 33%。MBR 中的优势菌为产碱杆菌(48.4%)和陶厄氏菌(15.2%)。此外,增强型 MBR 中脱氮基因(nirK、norB、nosZ)的丰度增加,有助于提高 TN 的去除效率。生物增效器的使用有效解决了污水处理中 HN-AD 损失的问题。

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Heterotrophic nitrification and aerobic denitrification characteristics of the psychrotolerant Pseudomonas peli NR-5 at low temperatures.耐冷性皮氏假单胞菌NR-5在低温下的异养硝化和好氧反硝化特性
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