黄海南部新型脱氮菌——宗洋林菌属的建立及其模式种黄海宗洋林菌的描述，同时将海洋滚动球菌归宗洋林菌属，更名为宗洋林菌海洋滚动球菌复合种。
Zongyanglinia huanghaiensis gen. nov., sp. nov., a novel denitrifying bacterium isolated from the yellow sea, and transfer of Pelagicola marinus to Zongyanglinia gen. nov. as Zongyanglinia marinus comb. nov.
机构信息
Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.
Department of Pathogenic Biology, Jining Medical University, Jining, 272067, People's Republic of China.
出版信息
Antonie Van Leeuwenhoek. 2021 Feb;114(2):137-149. doi: 10.1007/s10482-020-01507-1. Epub 2021 Jan 3.
Two Gram-stain-negative, oxidase- and catalase-positive, aerobic strains (CY05 and H18S-6) were isolated from sediment samples of the Yellow Sea, China. The strains were positive for denitrification. Optimum growth was observed at 20 °C, pH 7.5-8.0 and with 2.0%-3.0% NaCl. The predominant cellular fatty acids (> 10%) were summed feature 8 (C ω7c and/or C ω6c), major respiratory quinone was ubiquinone-10 and main polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and one unidentified aminolipid. The approximate genome size of strains CY05 and H18S-6 were 4.86 and 5.04 Mbp, the genomic G + C content of them were 54.2 and 54.5%, respectively. Both of the phylogenetic analysis based on 16S rRNA gene sequences and the up-to-date bacterial core gene (UBCG) sequences revealed that strains CY05, H18S-6 and Pelagicola marinus DSW4-44 formed a distinct monophyletic clade within the family Rhodobacteraceae. The ANI and isDDH values between strains CY05 and H18S-6 were 94.0% and 56.5%, between CY05 and Pelagicola marinus DSW4-44 were 94.1% and 59.8%, respectively, all below the accepted threshold value for species delineation. But the ANI and isDDH values between strains H18S-6 and Pelagicola marinus DSW4-44 were 96.8% and 76.7% respectively, indicating that strains H18S-6 and Pelagicola marinus DSW4-44 belong to the same species. Based on the distinctive polyphasic evidence, CY05 represent a novel species of a novel genus of the family Rhodobacteraceae, for which the name Zongyanglinia huanghaiensis gen. nov., sp. nov. is proposed. The type strain is CY05 (= MCCC 1K04409 = KCTC 62200). Moreover, the reclassification of Pelagicola marinus Choi et al. 2019 as Zongyanglinia marinus comb. nov. (type strain DSW4-44 = KCTC 62762 = KCCM 43261 = JCM 33637) is proposed based on the polyphasic taxonomic data obtained in this study.
两株革兰氏阴性、氧化酶和接触酶阳性、需氧菌株（CY05 和 H18S-6）从中国黄海沉积物样品中分离得到。这些菌株具有反硝化能力。在 20°C、pH 值为 7.5-8.0 和 2.0%-3.0%NaCl 的条件下生长最佳。主要细胞脂肪酸（>10%）为特征性 8（C ω7c 和/或 C ω6c），主要呼吸醌为泛醌-10，主要极性脂为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和一种未鉴定的胺脂。菌株 CY05 和 H18S-6 的近似基因组大小分别为 4.86 和 5.04 Mbp，基因组 G+C 含量分别为 54.2%和 54.5%。基于 16S rRNA 基因序列和最新细菌核心基因（UBCG）序列的系统发育分析表明，菌株 CY05、H18S-6 和 Pelagicola marinus DSW4-44 在红杆菌科内形成了一个独特的单系分支。菌株 CY05 和 H18S-6 之间的 ANI 和 isDDH 值分别为 94.0%和 56.5%，菌株 CY05 和 Pelagicola marinus DSW4-44 之间的 ANI 和 isDDH 值分别为 94.1%和 59.8%，均低于种系划分的可接受阈值。但是菌株 H18S-6 和 Pelagicola marinus DSW4-44 之间的 ANI 和 isDDH 值分别为 96.8%和 76.7%，表明菌株 H18S-6 和 Pelagicola marinus DSW4-44 属于同一物种。基于独特的多相证据，CY05 代表红杆菌科一个新属的新种，命名为 Zongyanglinia huanghaiensis gen. nov.，sp. nov.。模式菌株为 CY05（=MCCC 1K04409=KCTC 62200）。此外，根据本研究获得的多相分类学数据，提议将 Pelagicola marinus Choi 等人 2019 年的分类重新分类为 Zongyanglinia marinus comb. nov.（模式菌株 DSW4-44=KCTC 62762=KCCM 43261=JCM 33637）。
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