• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

旋转壁式容器系统中秋葵黏液的流动结构

Flow structure of okra mucilage in rotating wall vessel system.

作者信息

Zhu Weijun, Obara Hiromichi

机构信息

Tokyo Metropolitan University, Minamiosaw 1-1, Hachioji 192-0397, Tokyo, Japan.

出版信息

Heliyon. 2024 Aug 21;10(16):e36149. doi: 10.1016/j.heliyon.2024.e36149. eCollection 2024 Aug 30.

DOI:10.1016/j.heliyon.2024.e36149
PMID:39262968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11388502/
Abstract

The rotating-wall vessel (RWV) bioreactor, a 3D suspension culture system, faces challenges related to non-uniform tissue growth during the incubation of bone and heart tissues. Okra mucilage, an extract from okra pods with non-Newtonian rheological properties, has shown potential as a plasma replacement agent and has no induced cytotoxic effects. In this study, we investigated the flow structure of okra mucilage in rotating wall vessel system. By modifying the RWV and adding okra mucilage, we analyzed the flow structure using a high-speed camera and particle image velocimetry (PIV). Our results showed that okra mucilage creates a concentric circle-shaped rigid-like rotation at all rotation speeds (1-50 rpm). The high viscosity of okra mucilage resulted in a low terminal velocity for microparticles and quick response to rotational movements. These findings suggest that okra mucilage has the potential to enhance the uniformity of tissue growth in RWV systems by stabilizing the flow structure and reducing microparticle sedimentation.

摘要

旋转壁式容器（RWV）生物反应器是一种三维悬浮培养系统，在骨组织和心脏组织培养过程中面临着与组织生长不均匀相关的挑战。秋葵黏液是从秋葵荚中提取的具有非牛顿流变特性的物质，已显示出作为血浆替代剂的潜力，且无细胞毒性作用。在本研究中，我们研究了秋葵黏液在旋转壁式容器系统中的流动结构。通过对RWV进行改造并添加秋葵黏液，我们使用高速摄像机和粒子图像测速技术（PIV）分析了流动结构。我们的结果表明，秋葵黏液在所有转速（1 - 50转/分钟）下都会产生同心圆状的类似刚体的旋转。秋葵黏液的高粘度导致微粒的终端速度较低，并对旋转运动有快速响应。这些发现表明，秋葵黏液有可能通过稳定流动结构和减少微粒沉降来提高RWV系统中组织生长的均匀性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/1f2c1f97d1fa/gr010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/e8e1fed95fd2/gr001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/3f0ab6d439e4/gr002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/ed56e36184c9/gr003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/ed277680043d/gr004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/c62f228c83fc/gr005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/fb49c952d5df/gr006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/e4e62279d0e8/gr007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/3a4a815e2b73/gr008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/c6ca9cfd42f2/gr009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/1f2c1f97d1fa/gr010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/e8e1fed95fd2/gr001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/3f0ab6d439e4/gr002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/ed56e36184c9/gr003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/ed277680043d/gr004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/c62f228c83fc/gr005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/fb49c952d5df/gr006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/e4e62279d0e8/gr007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/3a4a815e2b73/gr008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/c6ca9cfd42f2/gr009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ced6/11388502/1f2c1f97d1fa/gr010.jpg

相似文献

1
Flow structure of okra mucilage in rotating wall vessel system.旋转壁式容器系统中秋葵黏液的流动结构
Heliyon. 2024 Aug 21;10(16):e36149. doi: 10.1016/j.heliyon.2024.e36149. eCollection 2024 Aug 30.
2
Investigation and prediction of the extensional viscosity of okra mucilage using the Giesekus model.利用 Giesekus 模型研究并预测黄秋葵胶的拉伸黏度。
Biorheology. 2023;59(1-2):29-42. doi: 10.3233/BIR-220006.
3
Indigenous Ethiopian okra ( mucilage: A novel ingredient with functional and antioxidant properties.埃塞俄比亚本土秋葵（黏液：一种具有功能和抗氧化特性的新型成分）。
Food Sci Nutr. 2018 Feb 2;6(3):563-571. doi: 10.1002/fsn3.596. eCollection 2018 May.
4
Experimental Study on the Application of Cellulosic Biopolymer for Enhanced Oil Recovery in Carbonate Cores under Harsh Conditions.在苛刻条件下纤维素生物聚合物用于提高碳酸盐岩岩心采收率的实验研究
Polymers (Basel). 2022 Oct 31;14(21):4621. doi: 10.3390/polym14214621.
5
Okra ( L.) as a Potential Functional Food Source of Mucilage and Bioactive Compounds with Technological Applications and Health Benefits.秋葵（L.）作为一种潜在的功能性食品来源，含有黏液质和生物活性化合物，具有技术应用价值和健康益处。
Plants (Basel). 2021 Aug 16;10(8):1683. doi: 10.3390/plants10081683.
6
Numerical simulation of microcarrier motion in a rotating wall vessel bioreactor.旋转壁式生物反应器中微载体运动的数值模拟
Biomed Environ Sci. 2006 Jun;19(3):163-8.
7
Extensional and shear rheology of okra polysaccharides in the presence of artificial saliva.在人工唾液存在下秋葵多糖的拉伸流变学和剪切流变学
NPJ Sci Food. 2018 Nov 20;2:20. doi: 10.1038/s41538-018-0029-1. eCollection 2018.
8
Microsphere formulations of ambroxol hydrochloride: influence of Okra (Abelmoschus esculentus) mucilage as a sustained release polymer.盐酸氨溴索微球制剂：秋葵（黄秋葵）黏液作为缓释聚合物的影响
Prog Biomater. 2020 Jun;9(1-2):65-80. doi: 10.1007/s40204-020-00132-5. Epub 2020 Jun 5.
9
Evaluation of in vitro antioxidant activity of okra mucilage and its antidiabetic and antihyperlipidemic effect in alloxan-induced diabetic mice.秋葵黏液的体外抗氧化活性及其对四氧嘧啶诱导的糖尿病小鼠的抗糖尿病和抗高血脂作用的评估。
Food Sci Nutr. 2021 Oct 28;9(12):6854-6865. doi: 10.1002/fsn3.2641. eCollection 2021 Dec.
10
Extensional and shear rheology of okra hydrocolloid-saliva mixtures.黄秋葵水溶胶-唾液混合物的拉伸和剪切流变学。
Food Res Int. 2018 Apr;106:204-212. doi: 10.1016/j.foodres.2017.12.051. Epub 2017 Dec 21.

引用本文的文献

1
Mucus-derived biomaterial dressings: a novel approach to accelerate wound healing.黏液衍生生物材料敷料：加速伤口愈合的新方法。
Theranostics. 2025 Jul 24;15(16):8068-8095. doi: 10.7150/thno.115988. eCollection 2025.

本文引用的文献

1
Activity pulses induce spontaneous flow reversals in viscoelastic environments.活性脉冲在黏弹性环境中诱导自发的流反转。
J R Soc Interface. 2021 Apr;18(177):20210100. doi: 10.1098/rsif.2021.0100. Epub 2021 Apr 14.
2
Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research.三维球体作为癌症研究的体外临床前模型
Pharmaceutics. 2020 Dec 6;12(12):1186. doi: 10.3390/pharmaceutics12121186.
3
Is It Time to Start Transitioning From 2D to 3D Cell Culture?是时候开始从二维细胞培养向三维细胞培养转变了吗？
Front Mol Biosci. 2020 Mar 6;7:33. doi: 10.3389/fmolb.2020.00033. eCollection 2020.
4
Clustering of instrumental methods to characterize the texture and the rheology of slimy okra (Abelmoschus esculentus) suspensions.仪器方法的聚类分析用于表征粘稠的秋葵（Abelmoschus esculentus）悬浮液的质地和流变学特性。
J Texture Stud. 2020 Jun;51(3):426-443. doi: 10.1111/jtxs.12505. Epub 2020 Jan 28.
5
Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells.球体培养系统方法及其在间充质干细胞中的应用。
Cells. 2019 Dec 12;8(12):1620. doi: 10.3390/cells8121620.
6
Effect of microgravity & space radiation on microbes.微重力和空间辐射对微生物的影响。
Future Microbiol. 2018 Jun 1;13:831-847. doi: 10.2217/fmb-2017-0251. Epub 2018 May 10.
7
In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions.在体秋葵（Abelmoschus esculentus）对乙醇诱导的急性胃黏膜损伤的抗溃疡作用。
Pharm Biol. 2018 Dec;56(1):165-175. doi: 10.1080/13880209.2018.1442481.
8
Polymeric microspheres of okra mucilage and alginate for the controlled release of oxcarbazepine: In vitro &in vivo evaluation.黄秋葵胶和海藻酸钠的聚合物微球用于奥卡西平的控制释放：体外和体内评价。
Int J Biol Macromol. 2018 May;111:1156-1165. doi: 10.1016/j.ijbiomac.2018.01.058. Epub 2018 Jan 12.
9
Three-Dimensional Cell Cultures in Drug Discovery and Development.三维细胞培养在药物发现和开发中的应用。
SLAS Discov. 2017 Jun;22(5):456-472. doi: 10.1177/1087057117696795.
10
Advances in multicellular spheroids formation.多细胞球体形成的进展。
J R Soc Interface. 2017 Feb;14(127). doi: 10.1098/rsif.2016.0877.