Ju Zhi-Hao, Liu Tian-Qing, Ma Xue-Hu, Cui Zhan-Feng
State Key Laboratory of Fine Chemical Engineering, Stem Cells & Tissue Engineering Laboratory, Dalian University of Technology, Liaoning, China.
Biomed Environ Sci. 2006 Jun;19(3):163-8.
To analyze the forces of rotational wall vessel (RWV) bioreactor on small tissue pieces or microcarrier particles and to determine the tracks of microcarrier particles in RWV bioreactor.
The motion of the microcarrier in the rotating wall vessel (RWV) bioreactor with both the inner and outer cylinders rotating was modeled by numerical simulation.
The continuous trajectory of microcarrier particles, including the possible collision with the wall was obtained. An expression between the minimum rotational speed difference of the inner and outer cylinders and the microcarrier particle or aggregate radius could avoid collisions with either wall. The range of microcarrier radius or tissue size, which could be safely cultured in the RWV bioreactor, in terms of shear stress level, was determined.
The model works well in describing the trajectory of a heavier microcarrier particle in rotating wall vessel.
分析旋转壁式生物反应器（RWV）对小组织块或微载体颗粒的作用力，并确定微载体颗粒在RWV生物反应器中的轨迹。
通过数值模拟对内外筒均旋转的旋转壁式生物反应器（RWV）中微载体的运动进行建模。
获得了微载体颗粒的连续轨迹，包括与壁面可能的碰撞情况。得出了内外筒最小转速差与微载体颗粒或聚集体半径之间的表达式，可避免与任一壁面碰撞。根据剪切应力水平确定了可在RWV生物反应器中安全培养的微载体半径或组织大小范围。
该模型在描述较重微载体颗粒在旋转壁式生物反应器中的轨迹方面效果良好。
