Wang Anqi, Cui Xiaohui, Shi Changsong
Department of Pediatric, People's Hospital of Henan University, Henan Provincial People's Hospital, Zhengzhou, China.
Department of Pediatric, Jiaozuo Women's and Children's Hospital, Jiaozuo, China.
Transl Pediatr. 2024 Aug 31;13(8):1336-1358. doi: 10.21037/tp-24-129. Epub 2024 Aug 28.
Diarrhea is the leading contributory factor of sickness and mortality among children under five and an economic burden for families. This study aimed to investigate the effects of mixed probiotics supplementation at different times (consecutive and alternate-hour) on intestinal microecology in Sprague-Dawley (SD) rats with acute diarrhea.
A total of 40 SD rats were randomly assigned to four groups, including the control group, model group, probiotic group A, and probiotic group B. An acute diarrhea model was induced by administration of 5% dextran sulfate sodium. Rats in probiotic group A and probiotic group B were fed with (), (), and () for a total of 7 days. Probiotic group A was fed with all probiotics simultaneously. Probiotic group B was fed with and simultaneously, and then after a 2-hour interval, with . Metagenomic next-generation sequencing was used to analyze the fecal samples from every rat. The metagenomic sequencing used in this experiment was used to evaluate the effect of probiotics on the composition as well as function of the gut microbiota in order to gain a deeper comprehension of probiotic-host interactions on health and disease.
The structure of the gut microbiota in probiotic group A showed significant changes. Compared to the model group, the abundance of some beneficial bacteria had increased, including (P=0.048), (P=0.050), and (P=0.042), and many opportunistic pathogenic bacteria has decreased, such as (P=0.001). Compared to the control group, the abundance of some beneficial bacteria had increased, including (P=0.02) and (P=0.002), and there was a reduction in the abundance of many opportunistic pathogenic bacteria such as (P=0.03), (P=0.009), and _sp_1-3 (P=0.002). In addition, metagenomic analysis showed that as well as an up-regulation of glycoside hydrolase expression, amino acid and inorganic ion transport, and metabolism-related pathways, there was a down-regulation of cell motility.
Simultaneous administration of probiotics may have more positive implications in improving the gut microbiota of acute diarrhea rats.
腹泻是五岁以下儿童患病和死亡的主要促成因素，也是家庭的经济负担。本研究旨在探讨不同时间（连续和间隔一小时）补充混合益生菌对急性腹泻的斯普拉格-道利（SD）大鼠肠道微生态的影响。
将40只SD大鼠随机分为四组，包括对照组、模型组、益生菌A组和益生菌B组。通过给予5%硫酸葡聚糖钠诱导急性腹泻模型。益生菌A组和益生菌B组大鼠分别喂食（）、（）和（），共7天。益生菌A组同时喂食所有益生菌。益生菌B组同时喂食和，间隔2小时后再喂食。采用宏基因组下一代测序分析每只大鼠的粪便样本。本实验中使用的宏基因组测序用于评估益生菌对肠道微生物群组成和功能的影响，以便更深入地理解益生菌与宿主在健康和疾病方面的相互作用。
益生菌A组的肠道微生物群结构发生了显著变化。与模型组相比，一些有益菌的丰度增加，包括（P = 0.048）、（P = 0.050）和（P = 0.042），许多机会致病菌减少，如（P = 0.001）。与对照组相比，一些有益菌的丰度增加，包括（P = 0.02）和（P = 0.002），许多机会致病菌的丰度降低，如（P = 0.03）、（P = 0.009）和_sp_1 - 3（P = 0.002）。此外，宏基因组分析表明，除了糖苷水解酶表达、氨基酸和无机离子转运以及代谢相关途径上调外，细胞运动性下调。
同时给予益生菌可能对改善急性腹泻大鼠的肠道微生物群有更积极的影响。
