Correggia Monica, Di Iorio Luciano, Bastianoni Alessia Benedicta, Yucel Mustafa, Cordone Angelina, Giovannelli Donato
Department of Biology, University of Naples Federico II, Naples, Italy.
Institute of Marine Sciences, Middle East Technical University, Mersin, Turkey.
Open Res Eur. 2024 Jul 29;3:94. doi: 10.12688/openreseurope.15605.3. eCollection 2023.
This standard operating procedure (SOP) describes an ion chromatography (IC) procedure for the major cations and anions in hydrothermal fluids. Hydrothermal fluids are aqueous solutions with a wide range of temperature, salinity, pH and ion species that can be used by microbial metabolism as electron donors and electron acceptors. Due to the high variability of the environmental physical-chemical parameters in these samples, we have developed this protocol taking into account the special features of the matrices analyzed. An Eco IC Metrohm system equipped with a conductivity detector was used. Calibration curves are linear in the 0.1 to 10 mg/L concentration range for cations Ca , Na , K , Mg , NH and anions Cl , Br , NO , NO , SO , PO .
本标准操作规程（SOP）描述了一种用于分析热液流体中主要阳离子和阴离子的离子色谱（IC）方法。热液流体是具有广泛温度、盐度、pH值和离子种类的水溶液，可被微生物代谢用作电子供体和电子受体。由于这些样品中环境物理化学参数的高度变异性，我们在考虑所分析基质特殊特征的基础上制定了本方案。使用配备了电导检测器的万通Eco IC系统。阳离子Ca、Na、K、Mg、NH 以及阴离子Cl、Br、NO 、NO 、SO 、PO 在0.1至10 mg/L浓度范围内校准曲线呈线性。
