Cochran M, Patterson D, Neoh S, Stevens B, Mazzachi R
Clin Chem. 1985 Aug;31(8):1314-6.
Gel filtration of plasma from hemodialysis patients, with use of reagents and apparatus with carefully minimized background Al concentrations, reproducibly showed a single peak for Al, corresponding exactly to the elution position of transferrin. The Al/transferrin molar ratio in adjacent fractions was constant (mean 0.126, SE 0.006) in replicate experiments. In contrast, the association of Al with albumin varied. Using both equilibrium dialysis and gel-filtration techniques, in the presence and absence of calcium or phosphate, we could demonstrate no significant binding of Al by human albumin at Al concentrations of 1 to 12 mumol/L. We saw no Al peak in pooled, concentrated, low-molecular-mass fractions of plasma gel-filtered on Sephadex G-50. Evidently, transferrin is the sole Al-binding protein in plasma of hemodialysis patients.
使用背景铝浓度经过精心最小化处理的试剂和仪器，对血液透析患者的血浆进行凝胶过滤，可重复性地显示铝出现单一峰，其洗脱位置与转铁蛋白完全对应。在重复实验中，相邻组分中铝与转铁蛋白的摩尔比恒定（平均值0.126，标准误0.006）。相比之下，铝与白蛋白的结合情况有所不同。采用平衡透析和凝胶过滤技术，无论有无钙或磷酸盐存在，在铝浓度为1至12μmol/L时，我们均未证明人白蛋白能与铝发生显著结合。在经葡聚糖凝胶G - 50凝胶过滤的血浆混合、浓缩、低分子量组分中，我们未观察到铝峰。显然，转铁蛋白是血液透析患者血浆中唯一的铝结合蛋白。
