Solbach W, Heeg K, Von Steldern D U, Röllinghoff M, Wagner H
Clin Exp Immunol. 1985 Jun;60(3):501-8.
The effect of cyclosporine A (CyA) on the expression of the Tac-antigen (IL-2 receptor) on PHA-activated PBMNC was analysed by immunofluorescence. In the initial experiments we determined the number of Tac(+) cells, disregarding cell size; we found that CyA did not affect the number of cells expressing the Tac antigen after lectin stimulation (Fig. 1, Table 1). When we found that comparable numbers of Tac(+)-lymphocytes absorbed less IL-2 when grown in CyA as compared to solvent controls, we analysed in more detail the correlation between Tac-antigen expression and cell size of cell populations grown in CyA. It was found that CyA prevented a majority of PHA-activated PBMNC to undergo blastogenesis despite having expressed the IL-2 receptor. A minority of the cells, however, were refractory to the CyA-mediated suppression of blast formation. Studies analysing the Tac antigen expression semiquantitatively showed that CyA reduced the intensity of Tac antigen expression on cells of all sizes.
通过免疫荧光分析了环孢素A（CyA）对PHA激活的外周血单个核细胞（PBMNC）上Tac抗原（白细胞介素-2受体）表达的影响。在最初的实验中，我们确定了Tac(+)细胞的数量，而不考虑细胞大小；我们发现，CyA不影响凝集素刺激后表达Tac抗原的细胞数量（图1，表1）。当我们发现与溶剂对照相比，相当数量的Tac(+)淋巴细胞在CyA中生长时吸收的白细胞介素-2较少时，我们更详细地分析了CyA中生长的细胞群体的Tac抗原表达与细胞大小之间的相关性。结果发现，尽管已经表达了白细胞介素-2受体，但CyA阻止了大多数PHA激活的PBMNC发生母细胞化。然而，少数细胞对CyA介导的母细胞形成抑制具有抗性。半定量分析Tac抗原表达的研究表明，CyA降低了所有大小细胞上Tac抗原表达的强度。
