关于环孢素A对白细胞介素-2受体表达的部分抑制作用及对凝集素诱导的淋巴母细胞形成的预防作用
On the partial suppression of IL-2 receptor expression and the prevention of lectin-induced lymphoblast formation by cyclosporine A.
作者信息
Solbach W, Heeg K, Von Steldern D U, Röllinghoff M, Wagner H
出版信息
Clin Exp Immunol. 1985 Jun;60(3):501-8.
The effect of cyclosporine A (CyA) on the expression of the Tac-antigen (IL-2 receptor) on PHA-activated PBMNC was analysed by immunofluorescence. In the initial experiments we determined the number of Tac(+) cells, disregarding cell size; we found that CyA did not affect the number of cells expressing the Tac antigen after lectin stimulation (Fig. 1, Table 1). When we found that comparable numbers of Tac(+)-lymphocytes absorbed less IL-2 when grown in CyA as compared to solvent controls, we analysed in more detail the correlation between Tac-antigen expression and cell size of cell populations grown in CyA. It was found that CyA prevented a majority of PHA-activated PBMNC to undergo blastogenesis despite having expressed the IL-2 receptor. A minority of the cells, however, were refractory to the CyA-mediated suppression of blast formation. Studies analysing the Tac antigen expression semiquantitatively showed that CyA reduced the intensity of Tac antigen expression on cells of all sizes.
通过免疫荧光分析了环孢素A（CyA）对PHA激活的外周血单个核细胞（PBMNC）上Tac抗原（白细胞介素-2受体）表达的影响。在最初的实验中，我们确定了Tac(+)细胞的数量，而不考虑细胞大小；我们发现，CyA不影响凝集素刺激后表达Tac抗原的细胞数量（图1，表1）。当我们发现与溶剂对照相比，相当数量的Tac(+)淋巴细胞在CyA中生长时吸收的白细胞介素-2较少时，我们更详细地分析了CyA中生长的细胞群体的Tac抗原表达与细胞大小之间的相关性。结果发现，尽管已经表达了白细胞介素-2受体，但CyA阻止了大多数PHA激活的PBMNC发生母细胞化。然而，少数细胞对CyA介导的母细胞形成抑制具有抗性。半定量分析Tac抗原表达的研究表明，CyA降低了所有大小细胞上Tac抗原表达的强度。
Zotero 插件