Calder V L, Bellamy A S, Owen S, Lewis C, Rudge P, Davison A N, Feldmann M
Department of Neurochemistry, Institute of Neurology, London, UK.
Clin Exp Immunol. 1987 Dec;70(3):570-7.
The effects of the immunosuppressant Cyclosporin A (CsA) on T cell activation in vivo and in vitro were examined using the monoclonal antibody, anti-Tac, for interleukin 2 (IL-2) receptors and 3.9C2, for a peptide fragment of human IL-2. Peripheral blood lymphocytes (PBL) were stimulated with PHA in the presence of CsA. The expression of IL-2 receptors and production of IL-2 were reduced. PBL from CsA-treated MS patients had significantly lower proportions of Tac+ cells compared with untreated patients. This inhibition was not reflected in the CSF lymphocyte populations from CsA-treated patients and indicates the urgent need for an immunosuppressant drug which can enter the CNS in sufficient concentrations to inhibit local T cell activation.
使用针对白细胞介素2（IL-2）受体的单克隆抗体抗Tac和针对人IL-2肽片段的3.9C2，研究了免疫抑制剂环孢素A（CsA）在体内和体外对T细胞活化的影响。在存在CsA的情况下，用PHA刺激外周血淋巴细胞（PBL）。IL-2受体的表达和IL-2的产生减少。与未治疗的患者相比，接受CsA治疗的多发性硬化症（MS）患者的PBL中Tac +细胞的比例明显更低。这种抑制作用在接受CsA治疗的患者的脑脊液淋巴细胞群体中并未体现，这表明迫切需要一种能够以足够浓度进入中枢神经系统以抑制局部T细胞活化的免疫抑制剂药物。
