Bloemena E, Van Oers M H, Weinreich S, Schellekens P T
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Clin Exp Immunol. 1988 Feb;71(2):308-13.
To elucidate the mechanisms of action of cyclosporin A (CyA) and prednisolone (pred), we investigated the effects of these immunosuppressive drugs on the expression of receptors for interleukin 2 (IL-2) as well as the effect of exogenous IL-2 on CyA-inhibited and pred-inhibited responses. Pred-induced inhibition of T cell proliferative responses could be reversed by addition of exogenous IL-2. In contrast, exogenous IL-2 could not overcome CyA-mediated inhibition of T cell proliferation. Receptors for IL-2, as measured by the expression of Tac antigen, were slightly decreased by CyA and pred. However, expression of the biologically active, high-affinity IL-2 receptors was not diminished. These data suggest that pred-mediated inhibition occurs at the level of the IL-2 system, whereas CyA affects the proliferative mechanism of the T cell itself.
为阐明环孢素A（CyA）和泼尼松龙（pred）的作用机制，我们研究了这些免疫抑制药物对白细胞介素2（IL-2）受体表达的影响，以及外源性IL-2对CyA抑制和pred抑制反应的影响。添加外源性IL-2可逆转pred诱导的T细胞增殖反应抑制。相反，外源性IL-2不能克服CyA介导的T细胞增殖抑制。通过Tac抗原的表达来衡量，IL-2受体略有减少，CyA和pred均可导致这种情况。然而，具有生物学活性的高亲和力IL-2受体的表达并未减少。这些数据表明，pred介导的抑制发生在IL-2系统水平，而CyA影响T细胞本身的增殖机制。
