Human Milk Bank, São Bernardo do Campo Women's Hospital, Santo Andre, Brazil.
Department of Paediatrics, Centro Universitario FMABC, Sao Paulo, Brazil.
Breastfeed Med. 2024 Nov;19(11):863-869. doi: 10.1089/bfm.2024.0249. Epub 2024 Sep 12.
This study aimed to analyze and compare the quantity of energy and fat using the infrared analysis and creamatocrit method in pasteurized human milk (HM) samples. This cross-sectional study analyzed 1,858 pasteurized human samples from 317 mothers at a single center. Infrared transmission spectrophotometry (Miris, Human Milk Analyser [HMA], Uppsala, Sweden) and the creamatocrit method were used to evaluate the quantity of energy and fat in pasteurized HM samples. The average age of donor mothers was 29.7 ± 5.1 years, and the median duration of lactation was 22 days (interquartile range [IQ]: 7.7-59.2). Full-term births were observed in 196 (95.1%) of the women. The values of energy (difference: +8.96 kcal/dL, 95% CI: 8.52-9.44 kcal/dL; < 0.001) and fat (difference: +0.40 g/dL, 95% CI: 0.35-0.45 g/dL; < 0.001) in HM samples obtained by Miris were higher than those by the creamatocrit method. The energy calculated and the fat measured by Miris in the HM samples correlated moderately and directly with the obtained by creamatocrit (fat, = 0.585; < 0.001 and energy, = 0.591; < 0.01). The linear regression, adjusted for maternal age and lactation time, showed that the energy values calculated by creamatocrit were directly associated with those of Miris (energy kcal/dL = 38.43 + [0.516 × kcal/dL of creamatocrit]). The energy and fat quantity of pasteurized HM samples obtained by the creamatocrit and infrared methods were significantly correlated. However, the values calculated by the creamatocrit method were significantly lower than those by the infrared analyzer.
本研究旨在通过红外线分析和比密离心法分析和比较巴氏消毒人乳（HM）样本中的能量和脂肪含量。这项横断面研究分析了来自单个中心的 317 位母亲的 1858 份巴氏消毒人乳样本。红外线透射分光光度法（Miris、Human Milk Analyser [HMA]、Uppsala、瑞典）和比密离心法用于评估巴氏消毒 HM 样本中的能量和脂肪含量。供体母亲的平均年龄为 29.7±5.1 岁，中位哺乳期为 22 天（四分位距 [IQR]：7.7-59.2）。196 名（95.1%）妇女为足月分娩。通过 Miris 获得的 HM 样本中的能量值（差值：+8.96 kcal/dL，95%CI：8.52-9.44 kcal/dL；<0.001）和脂肪值（差值：+0.40 g/dL，95%CI：0.35-0.45 g/dL；<0.001）高于比密离心法。Miris 计算的能量和测量的脂肪与比密离心法获得的能量和脂肪中度且直接相关（脂肪， = 0.585；<0.001 和能量， = 0.591；<0.01）。调整母亲年龄和哺乳期后，线性回归显示，比密离心法计算的能量值与 Miris 直接相关（能量 kcal/dL = 38.43 + [0.516 × 比密离心法的 kcal/dL]）。通过比密离心法和红外线方法获得的巴氏消毒 HM 样本中的能量和脂肪量显著相关。然而，比密离心法计算的能量值明显低于红外线分析仪。
