用于测量人乳脂肪含量的乳脂比容技术的可重复性。

Reproducibility of the creamatocrit technique for the measurement of fat content in human milk.

作者信息

Tie Wan Jun, Kent Jacqueline Coral, Tat Lai Ching, Rea Alethea, Hepworth Anna Rachel, Murray Kevin, Geddes Donna Tracy

机构信息

School of Molecular Sciences, The University of Western Australia, Perth, Western Australia, Australia.

Faculty of Engineering and Mathematical Sciences, Mathematics and Statistics, The University of Western Australia, Perth, Western Australia, Australia; College of Science, Health, Engineering and Education, Academic Operations, Information Technology, Mathematics and Statistics, Murdoch University, Perth, Western Australia, Australia.

出版信息

Food Chem. 2021 Sep 15;356:129708. doi: 10.1016/j.foodchem.2021.129708. Epub 2021 Mar 26.

DOI:10.1016/j.foodchem.2021.129708

PMID:33845253

Abstract

Quantification of human milk (HM) fat is important for determining the energy intake of infants. The simplest and most rapid method is the creamatocrit method. However, the reliability of the creamatocrit has not been comprehensively investigated. The aims of this study were to test the inter- and-intra-rater reliability of: 1) HM sampling after hand- or-machine mixing methods and 2) HM fat measurement by the creamatocrit method. Inter-and-intra rater HM sampling after hand- or-machine mixing methods had high intraclass correlation coefficient (>0.91). Inter-rater reliability of measurement of HM with low fat (<2%) resulted in high variability (median coefficient of variations (CVs) > 15%). Intra- and inter-rater reliability of measurement of HM with higher fat (>3.5%) had low variability (median CVs < 10%). As the greatest variation in the creamatocrit method occurred during the measurement of HM samples with low fat, duplicate readings are necessary to reduce discrepancies in every HM fat determination.

摘要

母乳（HM）脂肪的定量对于确定婴儿的能量摄入很重要。最简单、最快速的方法是乳脂比容法。然而，乳脂比容的可靠性尚未得到全面研究。本研究的目的是测试以下方面的评分者间和评分者内信度：1）手工或机器混合方法后的母乳采样；2）乳脂比容法测量母乳脂肪。手工或机器混合方法后的评分者间和评分者内母乳采样具有较高的组内相关系数（>0.91）。低脂肪（<2%）母乳测量的评分者间信度导致高变异性（变异系数（CV）中位数>15%）。高脂肪（>3.5%）母乳测量的评分者内和评分者间信度具有低变异性（CV中位数<10%）。由于乳脂比容法的最大变异发生在低脂肪母乳样品的测量过程中，因此每次测定母乳脂肪时都需要重复读数以减少差异。