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Establishment of an ELISA-based in vitro test system to quantify the major honey bee venom allergen Api m 10 in crude bee venoms and therapy allergen products.

作者信息

Landgraf Alisa, Hein Sascha, Paulus-Tremel Kathrin Elisabeth, Wolff Michelle Beatrice, Arend Meike, Junker Ann-Christine, Strecker Daniel, Döring Sascha, Völker Elke, Holzhauser Thomas, Schmidt Sandra, Kaul Susanne, Schülke Stefan, Mahler Vera

机构信息

Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany.

Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany.

出版信息

Allergy. 2025 Mar;80(3):861-864. doi: 10.1111/all.16313. Epub 2024 Sep 12.

DOI:10.1111/all.16313
PMID:39264014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11891423/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3565/11891423/bbd0ce51daa1/ALL-80-861-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3565/11891423/3ee7dff8662a/ALL-80-861-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3565/11891423/bbd0ce51daa1/ALL-80-861-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3565/11891423/3ee7dff8662a/ALL-80-861-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3565/11891423/bbd0ce51daa1/ALL-80-861-g001.jpg

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Establishment of an ELISA-based in vitro test system to quantify the major honey bee venom allergen Api m 10 in crude bee venoms and therapy allergen products.建立基于酶联免疫吸附测定(ELISA)的体外检测系统,以定量粗蜂毒和治疗性变应原产品中的主要蜜蜂毒液变应原Api m 10。
Allergy. 2025 Mar;80(3):861-864. doi: 10.1111/all.16313. Epub 2024 Sep 12.
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The honey bee venom allergen Api m 10 displays one major IgE epitope, Api m 10.蜜蜂毒液过敏原Api m 10呈现出一个主要的IgE表位,即Api m 10。
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Recombinant allergen-based IgE testing to distinguish bee and wasp allergy.基于重组变应原的 IgE 检测以区分蜜蜂和黄蜂过敏。
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本文引用的文献

1
Diagnosis and treatment of Hymenoptera venom allergy: S2k Guideline of the German Society of Allergology and Clinical Immunology (DGAKI) in collaboration with the Arbeitsgemeinschaft für Berufs- und Umweltdermatologie e.V. (ABD), the Medical Association of German Allergologists (AeDA), the German Society of Dermatology (DDG), the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery (DGHNOKC), the German Society of Pediatrics and Adolescent Medicine (DGKJ), the Society for Pediatric Allergy and Environmental Medicine (GPA), German Respiratory Society (DGP), and the Austrian Society for Allergy and Immunology (ÖGAI).膜翅目昆虫毒液过敏的诊断与治疗:德国变态反应与临床免疫学会(DGAKI)与职业与环境皮肤病学会(ABD)、德国变态反应科医生协会(AeDA)、德国皮肤病学会(DDG)、德国耳鼻咽喉头颈外科学会(DGHNOKC)、德国儿科学与青少年医学学会(DGKJ)、儿童变态反应与环境医学学会(GPA)、德国呼吸学会(DGP)以及奥地利变态反应与免疫学会(ÖGAI)联合发布的S2k指南
Allergol Select. 2023 Oct 2;7:154-190. doi: 10.5414/ALX02430E. eCollection 2023.
2
[Not Available].[无可用内容]
Allergo J. 2021;30(1):20-49. doi: 10.1007/s15007-020-4750-0. Epub 2021 Feb 12.
3
The Honeybee Venom Major Allergen Api m 10 (Icarapin) and Its Role in Diagnostics and Treatment of Hymenoptera Venom Allergy.蜜蜂毒液主要过敏原 Api m 10(Icarapin)及其在诊断和治疗膜翅目毒液过敏中的作用。
Curr Allergy Asthma Rep. 2020 Jun 16;20(9):48. doi: 10.1007/s11882-020-00943-3.
4
Shedding Light on the Venom Proteomes of the Allergy-Relevant Hymenoptera (European Paper Wasp) and spp. (Yellow Jacket).揭示与过敏相关的膜翅目昆虫(欧洲纸蜂)和 spp.(黄夹克)的毒液蛋白质组。
Toxins (Basel). 2020 May 14;12(5):323. doi: 10.3390/toxins12050323.
5
Predominant Api m 10 sensitization as risk factor for treatment failure in honey bee venom immunotherapy.主要的 Api m 10 致敏是蜜蜂毒液免疫治疗失败的风险因素。
J Allergy Clin Immunol. 2016 Dec;138(6):1663-1671.e9. doi: 10.1016/j.jaci.2016.04.024. Epub 2016 May 24.
6
IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology.通过蛋白质阵列技术评估蜜蜂(意大利蜜蜂)毒液过敏原Api m 10嵌合异构体的IgE识别。
Mol Immunol. 2015 Feb;63(2):449-55. doi: 10.1016/j.molimm.2014.09.018. Epub 2014 Oct 17.
7
Hymenoptera venom proteins and peptides for diagnosis and treatment of venom allergic patients.用于毒液过敏患者诊断和治疗的膜翅目毒液蛋白质和肽。
Inflamm Allergy Drug Targets. 2011 Oct;10(5):420-8. doi: 10.2174/187152811797200704.
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Api m 10, a genuine A. mellifera venom allergen, is clinically relevant but underrepresented in therapeutic extracts.Api m 10,一种真正的 A. mellifera 毒液过敏原,在临床中具有相关性,但在治疗性提取物中代表性不足。
Allergy. 2011 Oct;66(10):1322-9. doi: 10.1111/j.1398-9995.2011.02667.x. Epub 2011 Jun 10.
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Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein.新型IgE结合蜂毒蛋白icarapin的分子克隆与表达
FEBS Lett. 2006 Sep 4;580(20):4895-9. doi: 10.1016/j.febslet.2006.08.005. Epub 2006 Aug 10.