Medical School, Nantong University, Nantong, China.
Department of Burn and Plastic Surgery, Affiliated Hospital 2 of Nantong University, The First People's Hospital of Nantong, Nantong, China.
Wound Repair Regen. 2024 Nov-Dec;32(6):880-889. doi: 10.1111/wrr.13218. Epub 2024 Sep 12.
Diabetic foot ulcer (DFU) is a chronic and serious complication of diabetes mellitus. It is mainly caused by hyperglycaemia, diabetic peripheral vasculopathy and diabetic peripheral neuropathy. These conditions result in ulceration of foot tissues and chronic wounds. If left untreated, DFU can lead to amputation or even endanger the patient's life. Single-cell RNA sequencing (scRNA-seq) is a technique used to identify and characterise transcriptional subpopulations at the single-cell level. It provides insight into cellular function and the molecular drivers of disease. The objective of this paper is to examine the subpopulations, genes and molecules of cells associated with chronic wounds of diabetic foot by using scRNA-seq. The paper aims to explore the wound-healing mechanism of DFU from three aspects: inflammation, angiogenesis and extracellular matrix remodelling. The goal is to gain a better understanding of the mechanism of DFU wound healing and identify possible DFU therapeutic targets, providing new insights for the application of DFU personalised therapy.
糖尿病足溃疡（DFU）是糖尿病的一种慢性且严重的并发症。它主要由高血糖、糖尿病外周血管病和糖尿病周围神经病变引起。这些情况导致足部组织溃疡和慢性伤口。如果不加以治疗，DFU 可导致截肢甚至危及患者生命。单细胞 RNA 测序（scRNA-seq）是一种用于在单细胞水平上识别和描述转录亚群的技术。它提供了对细胞功能和疾病分子驱动因素的深入了解。本文的目的是通过 scRNA-seq 研究与糖尿病足慢性伤口相关的细胞亚群、基因和分子。本文旨在从炎症、血管生成和细胞外基质重塑三个方面探讨 DFU 的愈合机制。目的是更好地了解 DFU 伤口愈合的机制，并确定可能的 DFU 治疗靶点，为 DFU 个体化治疗的应用提供新的见解。
