Kaçar Selvi Ceren, Şenceol Barış, Erden Pınar Esra
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Türkiye.
Department of Chemistry, Polatlı Faculty of Science and Letters, Ankara Haci Bayram Veli University, Ankara, Türkiye.
Prep Biochem Biotechnol. 2025;55(3):309-317. doi: 10.1080/10826068.2024.2402340. Epub 2024 Sep 12.
This study reports the design and development of a disposable amperometric biosensor for the determination of L-glutamate. Glutamate oxidase (GlOx) was immobilized onto a screen-printed carbon electrode (SPE) modified with poly-L-Aspartic acid (PAsp), carbon quantum dots (CQD), and platinum nanoparticles (PtNP) for the construction of the biosensor. The surface composition of the modified SPE was optimized using the one variable at a time method. The morphological properties of the biosensor were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The electrochemical behavior of the modified electrodes was studied by cyclic voltammetry. Under the optimized experimental conditions the linear working range, detection limit and sensitivity of the GlOx/PtNP/CQD/PAsp/SPE were found to be 1.0 - 140 µM, 0.3 µM and 0.002 µA µM, respectively. The GlOx/PtNP/CQD/PAsp/SPE biosensor also exhibited good measurement repeatability. The as-developed biosensor was applied for the determination of L-glutamate in spiked serum samples and the average analytical recovery of added glutamate was 98.9 ± 3.9%.
本研究报告了一种用于测定L-谷氨酸的一次性安培生物传感器的设计与开发。将谷氨酸氧化酶（GlOx）固定在经过聚-L-天冬氨酸（PAsp）、碳量子点（CQD）和铂纳米颗粒（PtNP）修饰的丝网印刷碳电极（SPE）上，以构建生物传感器。采用一次改变一个变量的方法对修饰后的SPE的表面组成进行了优化。通过扫描电子显微镜和能量色散X射线光谱对生物传感器的形态学性质进行了表征。采用循环伏安法研究了修饰电极的电化学行为。在优化的实验条件下，GlOx/PtNP/CQD/PAsp/SPE的线性工作范围、检测限和灵敏度分别为1.0 - 140 μM、0.3 μM和0.002 μA μM。GlOx/PtNP/CQD/PAsp/SPE生物传感器还表现出良好的测量重复性。所开发的生物传感器用于加标血清样品中L-谷氨酸的测定，添加的谷氨酸的平均分析回收率为98.9±3.9%。
