Sensors Unit, Materials Division, IK4-CIDETEC, Parque Tecnológico de San Sebastián, Paseo Miramón 196, 20009 Donostia-San Sebastián, Spain.
Sensors Unit, Materials Division, IK4-CIDETEC, Parque Tecnológico de San Sebastián, Paseo Miramón 196, 20009 Donostia-San Sebastián, Spain.
Biosens Bioelectron. 2014 Jun 15;56:345-51. doi: 10.1016/j.bios.2014.01.047. Epub 2014 Jan 31.
A novel biosensor for lactate has been developed, using screen-printed carbon electrodes (SPCE) and lactate oxidase (LOx). The active surface of the electrodes was modified using a dispersion of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA) solution. In this way, sensitive, disposable, low cost and reliable hydrogen peroxide sensors were obtained. The immobilisation of LOx on top of these PtNp-CNF-PDDA/SPCEs resulted in amperometric biosensors with high operational stability. The sensitivity of the optimised lactate biosensor was 36.8 (mA/Mcm(2)) with a linear range of 25-1500 µM. The limit of detection was 11 µM (S/N=3). Reproducibility, selectivity and storage stability were also evaluated. Additionally, the stability of the biosensor was also predicted by a model based on thermal degradation. Finally, lactate in sweat and blood samples was determined in a sport test using LOx/PtNp-CNF-PDDA/SPCEs and commercial biosensors respectively. Based on these data, the validity of the sweat lactate for the determination of the lactate threshold is discussed.
已经开发出一种新型的乳酸生物传感器，使用丝网印刷碳电极（SPCE）和乳酸氧化酶（LOx）。通过在聚二烯丙基二甲基氯化铵（PDDA）溶液中分散铂纳米粒子修饰的碳纳米纤维（PtNp-CNF）来修饰电极的活性表面。通过这种方式，获得了灵敏、一次性、低成本和可靠的过氧化氢传感器。将 LOx 固定在这些 PtNp-CNF-PDDA/SPCE 上，得到了具有高操作稳定性的电流型生物传感器。优化后的乳酸生物传感器的灵敏度为 36.8（mA/Mcm(2)），线性范围为 25-1500 µM。检测限为 11 µM（S/N=3）。还评估了重现性、选择性和存储稳定性。此外，还通过基于热降解的模型预测了生物传感器的稳定性。最后，使用 LOx/PtNp-CNF-PDDA/SPCE 分别在运动测试中测定汗液和血液样本中的乳酸。基于这些数据，讨论了汗液乳酸用于确定乳酸阈值的有效性。
