Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Chongqing 400044, China.
Biomater Sci. 2024 Oct 22;12(21):5521-5533. doi: 10.1039/d4bm00859f.
The skin is the first natural barrier of the human body. Bacterial infections severely hinder the healing process of skin wounds and pose a great threat to human health. Therefore, it is particularly urgent to develop new antimicrobial strategies for bacterial pathogen clearance and wound healing. In this study, a metal-organic framework (MOF), Fe-MIL88B-NH, was incorporated with the photosensitizer indocyanine green (ICG) to construct composite nanoparticles (MOF@ICG NPs) with multiple antibacterial activities. Under mild near-infrared (NIR) irradiation, the photosensitizer ICG in the MOF@ICG NPs undergoes photothermal conversion (∼45 °C) and photodynamic reactions to generate heat and singlet oxygen (O). In addition, the Fenton reaction of the NPs with hydrogen peroxide (HO) in the bacterial infection microenvironment resulted in the generation of hydroxyl radicals (˙OH), thus achieving the three-mode combination of low-temperature photothermal therapy (PTT)/photodynamic therapy (PDT)/chemodynamic therapy (CDT). The experimental results showed that MOF@ICG MPs had excellent antibacterial properties and good cytocompatibility, with some ability to promote the migration of L-929 fibroblasts. Furthermore, under NIR irradiation, MOF@ICG NPs could significantly kill bacteria and promote skin wound healing according to the results of animal experiments. The wound healing rate reached 87.1% after 7 days of treatment. The research results break through the limitations of single-mode antibacterial technology and provide certain theoretical guidance and technical support for the research and development of new antibacterial materials.
皮肤是人体的第一道天然屏障。细菌感染严重阻碍皮肤伤口的愈合过程，对人类健康构成极大威胁。因此，开发新的抗菌策略以清除细菌病原体和促进伤口愈合尤为迫切。在本研究中，将金属-有机骨架（MOF）Fe-MIL88B-NH 与光敏剂吲哚菁绿（ICG）结合，构建具有多种抗菌活性的复合纳米粒子（MOF@ICG NPs）。在温和的近红外（NIR）照射下，MOF@ICG NPs 中的光敏剂 ICG 经历光热转换（约 45°C）和光动力反应，以产生热量和单线态氧（O）。此外，NP 在细菌感染微环境中与过氧化氢（HO）的芬顿反应导致羟基自由基（˙OH）的产生，从而实现低温光热疗法（PTT）/光动力疗法（PDT）/化学动力学疗法（CDT）的三模式组合。实验结果表明，MOF@ICG MPs 具有优异的抗菌性能和良好的细胞相容性，并且具有一定的促进 L-929 成纤维细胞迁移的能力。此外，在 NIR 照射下，根据动物实验结果，MOF@ICG NPs 可以显著杀死细菌并促进皮肤伤口愈合。经过 7 天的治疗，伤口愈合率达到 87.1%。研究结果突破了单一模式抗菌技术的局限性，为新型抗菌材料的研究和开发提供了一定的理论指导和技术支持。
