Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia.
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.
Bull Exp Biol Med. 2024 Aug;177(4):423-426. doi: 10.1007/s10517-024-06201-0. Epub 2024 Sep 12.
The level of ROS (fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate) and lipid content (fluorescent lipophilic dye Nile Red) in the peripheral blood monocyte fraction from patients with type 1 diabetes mellitus and healthy volunteers were assessed by flow cytofluorimetry. The number of CD36 monocytes was assessed using specific antibodies. In patients with type 1 diabetes mellitus, the levels of ROS and intracellular lipids in monocytes and the number of cells expressing CD36 fatty acid translocase were elevated. These results indicate metabolic changes in the peripheral blood cells of patients with carbohydrate metabolism disorders and can be considered as possible prognostic markers for the development of type 1 diabetes mellitus complications.
通过流式细胞荧光法评估了 1 型糖尿病患者和健康志愿者外周血单核细胞中 ROS（荧光探针 2'，7'-二氯二氢荧光素二乙酸酯）和脂质含量（荧光亲脂性染料尼罗红）的水平。使用特异性抗体评估了 CD36 单核细胞的数量。在 1 型糖尿病患者中，单核细胞中 ROS 和细胞内脂质的水平以及表达脂肪酸转运蛋白 CD36 的细胞数量均升高。这些结果表明碳水化合物代谢紊乱患者外周血细胞发生了代谢变化，可作为 1 型糖尿病并发症发展的可能预后标志物。
