Niemelä O, Risteli L, Sotaniemi E A, Risteli J
Eur J Clin Invest. 1985 Jun;15(3):132-7. doi: 10.1111/j.1365-2362.1985.tb00156.x.
The two major constituents of basement membranes are type IV collagen and laminin. Specific radioimmunoassays are described here for two structural domains of these proteins (7-S collagen and the fragment P1, respectively) that allow the related antigens to be quantified in human serum. The serum 7-S collagen antigen was uniform in size, whereas the laminin P1 antigenicity was heterogeneous. These proteins were measured in sera from sixty-three alcoholics, divided on the basis of liver histology into four groups: normal light microscopy, fatty liver, alcoholic cirrhosis with hepatitis and inactive cirrhosis. The group with cirrhosis and hepatitis had clearly elevated values in both assays, differing significantly from the others. A few pathological results were also seen in the other groups. The increases noted in 7-S collagen concentration were larger than those in laminin P1. During follow-up of a patient with cirrhosis and hepatitis the 7-S collagen level in particular seemed to reflect the course of the disease. The elevated basement membrane protein concentrations in serum may be associated with the formation of real basement membranes in the perisinusoidal space, a process known as capillarization of the sinusoids which is found during the development of liver cirrhosis.
基底膜的两个主要成分是IV型胶原蛋白和层粘连蛋白。本文描述了针对这些蛋白质的两个结构域（分别为7-S胶原蛋白和片段P1）的特异性放射免疫测定法，该方法可对人血清中的相关抗原进行定量。血清7-S胶原蛋白抗原大小均一，而层粘连蛋白P1抗原性具有异质性。在63名酗酒者的血清中检测了这些蛋白质，根据肝脏组织学将他们分为四组：正常光学显微镜检查、脂肪肝、伴有肝炎的酒精性肝硬化和静止性肝硬化。伴有肝硬化和肝炎的组在两种测定中值均明显升高，与其他组有显著差异。在其他组中也观察到一些病理结果。7-S胶原蛋白浓度的升高幅度大于层粘连蛋白P1。在一名伴有肝硬化和肝炎的患者随访期间，尤其是7-S胶原蛋白水平似乎反映了疾病的进程。血清中基底膜蛋白浓度升高可能与窦周隙中真正基底膜的形成有关，这一过程称为肝血窦毛细血管化，在肝硬化发展过程中会出现。
