Minimal quantities of FSH beta subunit are released by rat anterior pituitary cells in primary culture.

FSH beta in the medium and extracts of cultured rat anterior pituitary cells was quantitated with a heterologous radioimmunoassay utilizing anti-rFSH beta and hFSH beta as the standard and tracer. After 4 days in culture, extracts were prepared from washed cells, cells incubated for 6 h or 24 h in fresh medium or cells incubated for 6 h in the presence of 10(-7) M GnRH. FSH beta/rFSH molar ratios were approximately 0.05, 0.04, 0.21 and 0.35, respectively. The elevation in FSH beta/rFSH molar ratios in 24 h basal and GnRH-treated cultures was due in part to an increase in intracellular FSH beta. Both unstimulated and GnRH-treated cultures contained non-detectable quantities of FSH beta in the medium (less than 0.063 ng/100 000 cells; FSH beta/rFSH molar ratio less than 0.024). Thus, it appears that cultured rat anterior pituitary cells contain a small amount of uncombined FSH beta but that minimal quantities are released. Furthermore, GnRH may increase intracellular quantities of FSH beta but does not induce its release.