[使用恶臭假单胞菌PpG1的特定突变体通过烈性噬菌体pf16进行转导的有效方法]
[Effective method of transduction with virulent phage pf16 using specific mutants of Pseudomonas putida PpG1].
作者信息
Gorbunova S A, Akhverdian V S, Cheremukhina L V, Krylov V N
出版信息
Genetika. 1985 May;21(5):872-4.
PMID:3926609
Abstract
A procedure of simple selection of Pseudomonas putida PpG1 mutants is described. The mutants can be used for transduction with virulent pf16 phage, giving reliable results. The frequency of transduction of chromosomal markers ilv and trp was 10(-6). Also, transduction of plasmid RP4 with phage pf16 was shown with the frequency of 10(-7).
摘要
描述了一种简单筛选恶臭假单胞菌PpG1突变体的方法。这些突变体可用于用烈性噬菌体pf16进行转导，能得到可靠的结果。染色体标记ilv和trp的转导频率为10^(-6)。此外，还显示噬菌体pf16对质粒RP4的转导频率为10^(-7)。
Zotero 插件