Centre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, Queensland, 4811, Australia.
Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, Queensland, Australia.
Sci Data. 2024 Sep 12;11(1):999. doi: 10.1038/s41597-024-03840-w.
Abalone (family Haliotidae) are an ecologically and economically significant group of marine gastropods that can be found in tropical and temperate waters. To date, only a few Haliotis genomes are available, all belonging to temperate species. Here, we provide the first chromosome-scale abalone genome assembly and the first reference genome of the tropical abalone Haliotis asinina. The combination of PacBio long-read HiFi sequencing and Dovetail's Omni-C sequencing allowed the chromosome-level assembly of this genome, while PacBio Isoform sequencing across five tissue types enabled the construction of high-quality gene models. This assembly resulted in 16 pseudo-chromosomes spanning over 1.12 Gb (98.1% of total scaffolds length), N50 of 67.09 Mb, the longest scaffold length of 105.96 Mb, and a BUSCO completeness score of 97.6%. This study identified 25,422 protein-coding genes and 61,149 transcripts. In an era of climate change and ocean warming, this genome of a heat-tolerant species can be used for comparative genomics with a focus on thermal resistance. This high-quality reference genome of H. asinina is a valuable resource for aquaculture, fisheries, and ecological studies.
鲍鱼（鲍科）是一类在热带和温带水域中广泛分布的海洋腹足类动物，具有重要的生态和经济价值。迄今为止，仅有少数几个鲍科基因组可供使用，且均属于温带物种。本研究提供了第一个鲍科的染色体水平基因组组装和第一个热带鲍鱼 Haliotis asinina 的参考基因组。结合 PacBio 长读长 HiFi 测序和 Dovetail 的 Omni-C 测序，实现了该基因组的染色体水平组装，而在五个组织类型中进行的 PacBio Isoform 测序则能够构建高质量的基因模型。该组装生成了 16 个假染色体，跨越 1.12 Gb（总支架长度的 98.1%），N50 为 67.09 Mb，最长支架长度为 105.96 Mb，且 BUSCO 完整性评分为 97.6%。本研究共鉴定出 25422 个蛋白编码基因和 61149 个转录本。在气候变化和海洋变暖的时代，这个耐热物种的基因组可以用于与耐热性相关的比较基因组学研究。H. asinina 的这个高质量参考基因组是水产养殖、渔业和生态研究的宝贵资源。
