Murata H, Takahashi N, Yamada K
Histochem J. 1985 Feb;17(2):121-30. doi: 10.1007/BF01003212.
In a series of mammalian and avian tissues, asparagine-linked oligosaccharides of glycoproteins have been localized histochemically by means of combined periodic acid-Schiff (PAS) and almond glycopeptidase digestion procedures. According to the present results, the particular saccharide residues were found to be localized primarily in glycoproteins of connective and muscular tissue elements but were hardly visualizable or absent in glycoproteins of epithelial tissue elements. In view of the substrate specificity of almond glycopeptidase and the known staining selectivity of the PAS reaction, the majority of connective and muscular tissue elements are thought to be the main sites where plasma types of glycoproteins are involved.
在一系列哺乳动物和鸟类组织中，糖蛋白的天冬酰胺连接寡糖已通过高碘酸 - 席夫（PAS）与杏仁糖肽酶消化程序相结合的方法进行了组织化学定位。根据目前的结果，发现特定的糖残基主要定位于结缔组织和肌肉组织成分的糖蛋白中，而上皮组织成分的糖蛋白中则难以观察到或不存在。鉴于杏仁糖肽酶的底物特异性和已知的PAS反应染色选择性，大多数结缔组织和肌肉组织成分被认为是涉及血浆型糖蛋白的主要部位。
