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枯草芽孢杆菌Rec-突变体的营养细胞和芽孢的耐热性及耐紫外光性

Heat and UV light resistance of vegetative cells and spores of Bacillus subtilis Rec-mutants.

作者信息

Hanlin J H, Lombardi S J, Slepecky R A

出版信息

J Bacteriol. 1985 Aug;163(2):774-7. doi: 10.1128/jb.163.2.774-777.1985.

Abstract

The heat and UV light resistance of spores and vegetative cells of Bacillus subtilis BD170 (rec+) were greater than those of B. subtilis BD224 (recE4). Strain BD170 can repair DNA whereas BD224 is repair deficient due to the presence of the recE4 allele. Spores of a GSY Rec+ strain were more heat resistant than spores of GSY Rec- and Uvr- mutants. The overall level of heat and UV light resistance attained by spores may in part be determined by their ability to repair deoxyribonucleic acid after exposure to these two physical mutagens.

摘要

枯草芽孢杆菌BD170(rec +)的芽孢和营养细胞的耐热性和耐紫外光性高于枯草芽孢杆菌BD224(recE4)。BD170菌株能够修复DNA,而BD224由于存在recE4等位基因而缺乏修复能力。GSY Rec +菌株的芽孢比GSY Rec-和Uvr-突变体的芽孢更耐热。芽孢获得的耐热性和耐紫外光性的总体水平可能部分取决于它们在暴露于这两种物理诱变剂后修复脱氧核糖核酸的能力。

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本文引用的文献

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Bacillus subtilis "rec assay" test with isogenic strains.用同基因菌株进行枯草芽孢杆菌“rec测定”试验。
Appl Environ Microbiol. 1982 Jan;43(1):177-84. doi: 10.1128/aem.43.1.177-184.1982.
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Lethal heat induces single strand breaks in the DNA of bacterial spores.致死温度会导致细菌芽孢DNA出现单链断裂。
Biochem Biophys Res Commun. 1981 Jan 15;98(1):191-6. doi: 10.1016/0006-291x(81)91887-8.

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