Department of Biotechnology, Azarbaijan Shahid Madani University, Tabriz, Iran.
Mol Biotechnol. 2024 Jul;66(7):1563-1580. doi: 10.1007/s12033-023-00788-x. Epub 2023 Jun 20.
Agrobacterium tumefaciens-mediated plant transformation is the most dominant technique for the transformation of plants. It is used to transform monocotyledonous and dicotyledonous plants. A. tumefaciens apply for stable and transient transformation, random and targeted integration of foreign genes, as well as genome editing of plants. The Advantages of this method include cheapness, uncomplicated operation, high reproducibility, a low copy number of integrated transgenes, and the possibility of transferring larger DNA fragments. Engineered endonucleases such as CRISPR/Cas9 systems, TALENs, and ZFNs can be delivered with this method. Nowadays, Agrobacterium-mediated transformation is used for the Knock in, Knock down, and Knock out of genes. The transformation effectiveness of this method is not always desirable. Researchers applied various strategies to improve the effectiveness of this method. Here, a general overview of the characteristics and mechanism of gene transfer with Agrobacterium is presented. Advantages, updated data on the factors involved in optimizing this method, and other useful materials that lead to maximum exploitation as well as overcoming obstacles of this method are discussed. Moreover, the application of this method in the generation of genetically edited plants is stated. This review can help researchers to establish a rapid and highly effective Agrobacterium-mediated transformation protocol for any plant species.
农杆菌介导的植物转化是植物转化最主要的技术。它被用于转化单子叶和双子叶植物。农杆菌可用于稳定和瞬时转化、外源基因的随机和靶向整合，以及植物的基因组编辑。该方法的优点包括廉价、操作简单、重复性高、整合转基因的拷贝数低，以及转移较大 DNA 片段的可能性。该方法可递送工程内切酶，如 CRISPR/Cas9 系统、TALENs 和 ZFNs。如今，农杆菌介导的转化用于基因的敲入、敲低和敲除。该方法的转化效果并不总是理想的。研究人员应用了各种策略来提高该方法的效果。本文对农杆菌基因转移的特点和机制进行了概述。讨论了该方法的优点、优化该方法所涉及的因素的最新数据，以及其他有助于最大限度利用该方法并克服该方法障碍的有用材料。此外，还陈述了该方法在遗传编辑植物生成中的应用。本综述可以帮助研究人员为任何植物物种建立快速、高效的农杆菌介导的转化方案。
