College & Hospital of Stomatology, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei, 230032, China.
Department of Periodontology, College & Hospital of Stomatology, Anhui Medical University, Hefei, 230032, China.
J Mater Chem B. 2024 Oct 9;12(39):9938-9946. doi: 10.1039/d4tb01658k.
Barrier membranes have been used for the treatment of alveolar bone loss caused by periodontal diseases or trauma. However, an optimal barrier membrane must satisfy multiple requirements simultaneously, which are challenging to combine into a single material. We herein report the design of a bioinspired membrane consisting of three functional layers. The primary layer is composed of clay nanosheets and chitin, which form a nacre-inspired laminated structure. A calcium phosphate mineral layer is deposited on the inner surface of the nacre-inspired layer, while a poly(lactic acid) layer is coated on the outer surface. The composite membrane integrates good mechanical strength and deformability because of the nacre-inspired structure, facilitating operations during the implant surgery. The mineral layer induces the osteogenic differentiation of bone marrow mesenchymal stem cells and increases the stiffness of the membrane, which is an important factor for the regeneration process. The poly(lactic acid) layer can prevent unwanted mineralization on the outer surface of the membrane in oral environments. Cell experiments reveal that the membrane exhibits good biocompatibility and anti-infiltration capability toward connective tissue/epithelium cells. Furthermore, analyses show that the membrane does not degrade too fast, allowing enough time for bone regeneration. experiments prove that the membrane can effectively induce better bone regeneration and higher trabecular bone density in alveolar bone defects. This study demonstrates the potential of this bioinspired triple-layered membrane with hierarchical structures as a promising barrier material for periodontal guided tissue regeneration.
屏障膜已被用于治疗牙周病或创伤引起的牙槽骨丧失。然而，理想的屏障膜必须同时满足多个要求，这很难结合到一种材料中。本文报告了一种由三个功能层组成的仿生膜的设计。主要层由粘土纳米片和壳聚糖组成，形成珍珠层启发的层状结构。在珍珠层启发层的内表面上沉积钙磷矿物层，而在其外表面上涂覆聚乳酸层。由于具有珍珠层启发的结构，复合膜具有良好的机械强度和可变形性，便于在植入手术中操作。矿物层可诱导骨髓间充质干细胞的成骨分化并增加膜的刚度，这是再生过程中的一个重要因素。聚乳酸层可以防止在口腔环境中外层膜发生不需要的矿化。细胞实验表明，该膜具有良好的生物相容性和对结缔组织/上皮细胞的抗渗透能力。此外，分析表明该膜降解速度不会过快，从而为骨再生提供足够的时间。体内实验证明，该膜可有效诱导牙槽骨缺损中更好的骨再生和更高的小梁骨密度。本研究证明了这种具有分层结构的仿生三层膜作为一种有前途的牙周组织引导再生屏障材料的潜力。
