Yoshimoto Itsumi, Sasaki Jun-Ichi, Tsuboi Ririko, Yamaguchi Satoshi, Kitagawa Haruaki, Imazato Satoshi
Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Dent Mater. 2018 Mar;34(3):538-550. doi: 10.1016/j.dental.2017.12.011. Epub 2018 Jan 5.
Various commercial products are available for guided tissue regeneration (GTR) therapy; however, they do not combine biosafety with the ability to control cell function. The purpose of this study was to evaluate the physicochemical and biological characteristics of the novel bilayer biodegradable poly(lactic-co-glycolic acid) (PLGA) membrane, and to assess whether the bilayer PLGA membrane could be used for periodontal tissue regeneration.
Bilayer biodegradable membrane was fabricated thorough a two-step freezing and lyophilization process using PLGA solution. The characteristics of bilayer membranes were evaluated with respect to surface morphology, stability, mechanical strength, and operability for clinical use. Cell proliferation and osteogenic differentiation were investigated on the each surface of bilayer membrane. Then, these membranes were implanted to the rat calvaria bone defect models and evaluated their capability for tissue regeneration.
Biodegradable membranes composed of the solid and porous layer were successfully prepared and the surface morphologies analyzed. Physicochemical analyses revealed that the membranes possessed enough stability and mechanical properties for clinical use. It was also confirmed that the solid layer inhibited cell proliferation and subsequent connective tissue invasion, while the inner layer promoted proliferation and osteogenic differentiation, thus resulting in bone regeneration in vivo.
The layering technology used to fabricate the bilayer polymer membrane could be applied in the developing of other novel biomaterials. The present study demonstrates that the bilayer biodegradable polymer membranes facilitate tissue regeneration in vivo, and therefore represent a prospective biomaterial for GTR therapy.
有多种商业产品可用于引导组织再生（GTR）治疗；然而，它们并未将生物安全性与控制细胞功能的能力相结合。本研究的目的是评估新型双层可生物降解聚乳酸 - 乙醇酸共聚物（PLGA）膜的物理化学和生物学特性，并评估双层PLGA膜是否可用于牙周组织再生。
使用PLGA溶液通过两步冷冻和冻干过程制备双层可生物降解膜。从表面形态、稳定性、机械强度和临床使用的可操作性方面评估双层膜的特性。在双层膜的每个表面上研究细胞增殖和成骨分化。然后，将这些膜植入大鼠颅骨骨缺损模型中，并评估它们的组织再生能力。
成功制备了由固体层和多孔层组成的可生物降解膜，并分析了其表面形态。物理化学分析表明，这些膜具有足够的稳定性和机械性能以供临床使用。还证实，固体层抑制细胞增殖和随后的结缔组织侵入，而内层促进增殖和成骨分化，从而在体内导致骨再生。
用于制造双层聚合物膜的分层技术可应用于其他新型生物材料的开发。本研究表明，双层可生物降解聚合物膜在体内促进组织再生，因此代表了一种用于GTR治疗的前瞻性生物材料。
