Jadidi Aliakbar, Ghasemian Abdolmajid, Abdollahi Abbas, Abbasi-Dokht Tannaz, Abdollahzadeh Esmail, Baharlou Rasoul
Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran.
Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.
Iran J Microbiol. 2024 Aug;16(4):536-544. doi: 10.18502/ijm.v16i4.16313.
The consumption of contaminated poultry meat is considered as a significant route of campylobacteriosis transmission. Lactic acid is a disinfectant agent with bactericidal effects on spp. The purpose of this study was to assess the low concentrations of lactic acid effect and different temperatures on the transcriptomic responses of adhesion and virulence-associated genes including and .
The samples were incubated at 10°C and 22°C for 48 h upon exposure to 30% and 60% lactic acid. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lactic acid was also determined. Then, gene expression was assessed using real-time polymerase chain reaction (RT-PCR).
Lactic acid had lower MIC and MBC levels at lower temperature. The utilization of both levels of lactic acid significantly reduced the expression of and genes over 48 h of incubation at 22°C. However, no significant difference was found in the expression of the gene between 10 and 22°C at 30% lactic acid.
These results highlight the potential of low-concentration lactic acid in the downregulation of adhesion and virulence-associated genes as well as reduction of pathogenicity.
食用受污染的禽肉被认为是弯曲菌病传播的重要途径。乳酸是一种对弯曲菌具有杀菌作用的消毒剂。本研究的目的是评估低浓度乳酸效应以及不同温度对包括空肠弯曲菌和结肠弯曲菌在内的弯曲菌黏附及毒力相关基因转录组反应的影响。
样本在暴露于30%和60%乳酸后，于10°C和22°C孵育48小时。还测定了乳酸的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。然后，使用实时聚合酶链反应（RT-PCR）评估基因表达。
乳酸在较低温度下具有较低的MIC和MBC水平。在22°C孵育48小时期间，两种乳酸水平的使用均显著降低了空肠弯曲菌和结肠弯曲菌基因的表达。然而，在30%乳酸条件下，10°C和22°C之间空肠弯曲菌基因的表达未发现显著差异。
这些结果突出了低浓度乳酸在下调黏附及毒力相关基因以及降低弯曲菌致病性方面的潜力。
