Effect of essential oil on A efflux pump gene expression and biofilm formation in ciprofloxacin-resistant strains.

BACKGROUND AND OBJECTIVES

Today, medicinal plants and their derivatives are considered to reduce the prevalence of antibiotic resistance. The aim of this study was to investigate the effect of essential oil on A efflux pump gene expression and biofilm formation in ciprofloxacin-resistant strains.

MATERIALS AND METHODS

A total of 50 clinical strains of resistant to ciprofloxacin were studied. The minimum inhibitory concentration (MIC) of essential oil and its synergistic effect with ciprofloxacin were determined using the microbroth dilution method and the fractional inhibitory concentration (FIC) method. Minimum biofilm inhibition concentration (MBIC) of essential oil was detected. The effect of essential oils on the expression level of the A gene was detected by Real-time PCR.

RESULTS

essential oil showed inhibitory activity against ciprofloxacin-resistant strains of When essential oil was combined with ciprofloxacin, the MIC was reduced 2-4 times. In 28% of the strains, with ciprofloxacin showed a synergistic effect. essential oil reduces the strength of biofilm formation and alters the biofilm phenotype. A significant decrease in A gene expression was observed in all isolates after treatment with essential oil.

CONCLUSION

Based on the results of this study, it was observed that supplementing essential oil can help reduce ciprofloxacin resistance and inhibit biofilm formation in fluoroquinolone-resistant strains.