Pazarci Ozhan, Tutar Ugur, Kilinc Seyran
Department of Orthopaedics and Traumatology, Cumhuriyet University School of Medicine, Sivas, Turkey.
Department of Nutrition and Dietetics, Cumhuriyet University of Health Sciences, Sivas, Turkey.
Eurasian J Med. 2019 Jun;51(2):128-132. doi: 10.5152/eurasianjmed.2019.18432.
This study aimed to determine the antibiofilm activity of essential oil (EO) against biofilms forming on in-vitro implant surfaces.
and biofilms were used. Stainless steel and titanium samples were grouped as control, water diluted, no EO addition, and reducing amounts of EO doses. The six microorganisms included in the study were investigated to examine if there were differences between the doses on the implant surfaces. The eradication effect of the EO in samples investigated with electron microscope was classified as 0: none, 1: mild, 2: moderate, and 3: severe. The chemical composition of the EO was determined with gas chromatography.
In terms of biofilm formation, no difference was observed between implant surfaces. While and were observed to be the most susceptible, was identified as the most resistant. According to gas chromatography, EO comprised 61.40% carvacrol and 0.28% thymol.
In vitro, EO was shown to be effective against gram negative/positive and fungal biofilms forming on the surface of stainless steel and titanium implants.
本研究旨在确定精油（EO）对体外植入物表面形成的生物膜的抗生物膜活性。
使用了不锈钢和钛样本以及生物膜。不锈钢和钛样本分为对照组、用水稀释组、不添加EO组以及EO剂量递减组。对研究中包含的六种微生物进行调查，以检查植入物表面不同剂量之间是否存在差异。用电子显微镜观察样本中EO的根除效果分为0级：无，1级：轻度，2级：中度，3级：重度。通过气相色谱法测定EO的化学成分。
在生物膜形成方面，未观察到植入物表面之间存在差异。观察到[具体微生物1]和[具体微生物2]最易受影响，而[具体微生物3]被确定为最具抗性。根据气相色谱法，EO含有61.40%的香芹酚和0.28%的百里酚。
在体外，EO对在不锈钢和钛植入物表面形成的革兰氏阴性/阳性和真菌生物膜显示出有效性。
