Department of Microbiology, Faculty of Medicine, Medical Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.
School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
BMC Complement Med Ther. 2020 Mar 19;20(1):92. doi: 10.1186/s12906-020-02887-7.
Acinetobacter baumannii is an opportunistic pathogen that causes nosocomial infections especially in patients in intensive care units (ICUs). Accordingly, the aim of our study was to detection of adeABC efllux pump encoding genes and antimicrobial effect of the essential oil of Mentha longifolia and Menthol on the minimum inhibitory concentration (MIC) of imipenem and ciprofloxacin in clinical isolates of A. baumannii.
A total of 75 clinical isolates of A. baumannii were collected. The presence of efflux pump genes was detected by polymerase chain reaction (PCR). The minimum inhibitory concentration (MIC) of the essential oil of Mentha longifolia and Menthol and their combined effect with antibiotics were measured by microbroth dilution method and fractional inhibitory concentration (FIC) index.
The frequency of adeA, adeB, and adeC genes in clinical isolates of A. baumannii were 86.7, 90.7, and 92%, respectively. When the essential oil of Mentha longifolia was combined with ciprofloxacin and imipenem, MICs decreased 4- and 8-fold, respectively. In the combination of menthol with imipenem, the resistance to imipenem was reduced from 0- to 16-fold in 90% (63/70) of the isolates.
The presence of efflux pump genes in more than 90% of A. baumannii isolates indicates its potential role in inducing imipenem- and ciprofloxacin-resistance in this bacterium. Menthol has an antimicrobial effect as an active ingredient in Mentha longifolia. In the future, the combination of medicinal plants with antibiotics can be used as a complement in treating diseases caused by drug-resistant bacteria such as A. baumannii infections.
鲍曼不动杆菌是一种机会性病原体，尤其会导致重症监护病房（ICU）患者发生医院感染。因此，我们的研究目的是检测鲍曼不动杆菌中 adeABC 外排泵编码基因，并研究唇形科薄荷属长叶薄荷精油和薄荷醇对临床分离株中亚胺培南和环丙沙星的最小抑菌浓度（MIC）的影响。
共收集 75 株临床分离的鲍曼不动杆菌。采用聚合酶链反应（PCR）检测外排泵基因的存在。采用微量肉汤稀释法和部分抑菌浓度（FIC）指数测定长叶薄荷精油和薄荷醇的最小抑菌浓度（MIC）及其与抗生素的联合作用。
临床分离的鲍曼不动杆菌 adeA、adeB 和 adeC 基因的频率分别为 86.7%、90.7%和 92%。当长叶薄荷精油与环丙沙星和亚胺培南联合使用时，MIC 值分别降低了 4 倍和 8 倍。在薄荷醇与亚胺培南联合使用时，90%（63/70）的分离株对亚胺培南的耐药性从 0 倍降低至 16 倍。
超过 90%的鲍曼不动杆菌分离株中存在外排泵基因，表明其在外排泵基因诱导该细菌对亚胺培南和环丙沙星耐药中发挥重要作用。薄荷醇作为长叶薄荷的有效成分具有抗菌作用。未来，植物药与抗生素联合使用可以作为治疗鲍曼不动杆菌等耐药菌引起的疾病的一种补充方法。
