Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Institute of Feed Science, College of Animal Science, Zhejiang University, Hangzhou, China.
Microbiol Immunol. 2012 Dec;56(12):817-24. doi: 10.1111/j.1348-0421.2012.00508.x.
To investigate the immunomodulatory effects of Bacillus subtilis (B. subtilis) (natto) B4 spores on murine macrophage, RAW 264.7 cells were cultured alone or with B subtilis (natto) B4 spores at 37°C for 12 hrs, then both cells and culture supernatants were collected for analyses. Exposure of RAW 264.7 cells to B. subtilis (natto) B4 spores had no significant effects on macrophage viability and amounts of extracellular lactate dehydrogenase (LDH). However, it remarkably increased the activities of acid phosphatase (ACP), lactate dehydrogenase (LDH) and inducible nitric oxide synthase (iNOS) in cells and the amounts of nitric oxide (NO) and cytokines (tumor necrosis factor-alpha, interferon-gamma, interleukin [IL]-1 beta, IL-6, IL-12, IL-10 and macrophage inflammatory protein-2) in culture supernatants. These results demonstrate that B. subtilis (natto) B4 spores are harmless to murine macrophages and can stimulate their activation through up-regulation of ACP and LDH activities and enhance their immune function by increasing iNOS activity and stimulating NO and cytokine production. The above findings suggest that B. subtilis (natto) B4 spores have immunomodulatory effects on macrophages.
为了研究枯草芽孢杆菌（纳豆菌）B4 孢子对鼠源巨噬细胞 RAW264.7 的免疫调节作用，将 RAW264.7 细胞单独培养或与枯草芽孢杆菌（纳豆菌）B4 孢子一起在 37°C 下培养 12 小时，然后收集细胞和培养上清液进行分析。暴露于枯草芽孢杆菌（纳豆菌）B4 孢子的 RAW264.7 细胞对巨噬细胞活力和细胞外乳酸脱氢酶（LDH）的含量没有显著影响。然而，它显著增加了细胞中酸性磷酸酶（ACP）、乳酸脱氢酶（LDH）和诱导型一氧化氮合酶（iNOS）的活性以及培养上清液中一氧化氮（NO）和细胞因子（肿瘤坏死因子-α、干扰素-γ、白细胞介素[IL]-1β、IL-6、IL-12、IL-10 和巨噬细胞炎症蛋白-2）的含量。这些结果表明，枯草芽孢杆菌（纳豆菌）B4 孢子对鼠源巨噬细胞无害，可通过上调 ACP 和 LDH 活性刺激其活化，并通过增加 iNOS 活性和刺激 NO 和细胞因子产生来增强其免疫功能。上述发现表明，枯草芽孢杆菌（纳豆菌）B4 孢子对巨噬细胞具有免疫调节作用。
