巴氏杀菌可通过调节 TLR2 介导的 AMPK 和 NF-κB 改善 LPS 诱导的 Caco-2 细胞肠屏障功能障碍。

Pasteurized Ameliorate the LPS-Induced Intestinal Barrier Dysfunction via Modulating AMPK and NF-κB through TLR2 in Caco-2 Cells.

机构信息

National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruits and Vegetables Processing Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Beijing DaBeiNong Biotechnology Co., Ltd., Beijing 100193, China.

出版信息

Nutrients. 2022 Feb 11;14(4):764. doi: 10.3390/nu14040764.

is well known for the amelioration of inflammatory responses and restoration of intestinal barrier function. The beneficial effect of occurred through contacting Toll-like receptor 2 (TLR2) on intestinal epithelial cells by wall components. In this case, the downstream mechanism of pasteurized stimulating TLR2 for ameliorated intestinal barrier function is worth investigating. In this study, we evaluated the effect of live and pasteurized on protecting the barrier dysfunction of Caco-2 intestinal epithelial cells induced by lipopolysaccharide (LPS). We discovered that both live and pasteurized could attenuate an inflammatory response and improve intestinal barrier integrity in Caco-2 monolayers. We demonstrated that enhances AMP-activated protein kinase (AMPK) activation and inhibits Nuclear Factor-Kappa B (NF-κB) activation through the stimulation of TLR2. Overall, we provided a specific mechanism for the probiotic effect of on the intestinal barrier function of Caco-2 cells.

具有改善炎症反应和恢复肠道屏障功能的作用。这种有益的效果是通过壁成分与肠道上皮细胞上的 Toll 样受体 2（TLR2）相互作用而产生的。在这种情况下，值得研究巴氏杀菌对 TLR2 刺激以改善肠道屏障功能的下游机制。在这项研究中，我们评估了活菌和巴氏杀菌对保护 Caco-2 肠上皮细胞中由脂多糖（LPS）诱导的屏障功能障碍的影响。我们发现活菌和巴氏杀菌都可以减轻 Caco-2 单层中的炎症反应并改善肠道屏障完整性。我们证明，通过刺激 TLR2，增强了 AMP 激活的蛋白激酶（AMPK）的激活并抑制了核因子-κB（NF-κB）的激活。总的来说，我们为对 Caco-2 细胞肠道屏障功能的益生菌作用提供了一个特定的机制。